参附益心颗粒对慢性心力衰竭大鼠心肌血管紧张素Ⅱ1型受体和2型受体表达的影响

Effects of Shenfuyixin Granule on myocardial angiotensin II type 1 receptor and type 2 receptor's expression of chronic heart failure rats

目的:观察参附益心颗粒对心衰大鼠心肌血管紧张素Ⅱ1型受体和2型受体(AT1和AT2)表达的影响。方法:随机将150只雄性SD大鼠分为造模组130只与假手术组20只,结扎大鼠左冠状动脉前降支(LAD)建立心衰大鼠模型,假手术组手术方法同上,仅在LAD下穿线不结扎。造模4周后检测左心室射血分数(LVEF)<50%者随机分为5组,每组10只,分别为卡托普利组、氯沙坦组、参附益心颗粒高、低剂量组、模型组,另选假手术组大鼠10只。假手术组及模型组以纯净水灌胃,药物干预组以药物溶液灌胃给药,每天1次,4周后查LVEF、称重后取标本,免疫组化法检测大鼠心肌AT1、AT2表达。结果:参附益心颗粒能明显提高心衰大鼠LVEF。心衰大鼠AT1、AT2表达显著增强,参附益心颗粒可明显降低AT1、AT2表达。结论:参附益心颗粒可改善心衰大鼠心功能,下调心肌中AT1、AT2表达水平阻断肾素血管紧张素系统,可能会起到延缓或改善心肌重塑的作用。

Objective： To study the effect of Shenfuyixin Granule on the expression of myocardial angiotensin 1I type 1 receptor and type 2 receptor （AT1 and AT2） in heart failure rats. Methods： 150 heathly male SD rats were randomly divided into sham-operated group （n=20） and model group （n=130）. In model group the left anterior descending coronary artery was occluded, in sham operation group the sutures were passed under the left anterior descending coronary artery without ligation. After 4 weeks the modeling group were randomly assigned to 5 groups （n=10） based on the value of LVEF〈50% obtained by Color Doppler Ultrasound, such as captopril group, losartan group, two groups of Shenfuyixin Granule （low dose, high dose）, model group. All the medicine was given through gavage once a day for 4 weeks, the rats in model group and sham operation group （n=10） were administered gavage with equivalent sodium chloride once a day for 4 weeks. After 4 weeks The LVEF was valued again with Color Doppler Ultrasound; the tissue was collected to detect index. Results： The LVEF was improved markedly in Shenfuyixin Granule. Two Shenfuyixin Granule groups obviously decreased myocardial AT1 and AT2. Conclusion： Shenfuyixin Granule can improve Cardiac function of CHF rats, decrease myocardial AT1 and AT2 to block RAS, maybe delay or improve cardiac remodeling.