复方中药益气祛白方对蘑菇酪氨酸酶活性及黑素生成的影响

Effects of extract of Yiqiqubai Compound on mushroom tyrosinase activity and melanogenesis

目的:运用血清药理学方法研究益气祛白方对体外酪氨酸酶(TYR)活性及多巴色素自动氧化生成黑素量的影响。方法:分别以益气祛白方的水提物与醇提物制备含药血清,体外观察其对于TYR活性及多巴色素自动氧化生成黑素量的影响。结果:益气祛白方水提物及醇提物均可以明显提高TYR活性和多巴色素自动氧化生成的黑素量,与正常对照组比较,差异具有显著意义(P<0.05),其中益气祛白方水提组效果更好。结论:益气祛白方对TYR活性和多巴色素自动氧化生成黑素的上调作用在白癜风的治疗方面具有积极意义。

Objective： To study the effects of TCM Yiqiqubai Compound on mushroom tyrosinase activity and melanogenesis by serum pharmacological method. Methods： The medicated serum of mice was prepared from aqueous extract and ethanol soluble extract of Yiqiqubai Compound. The effects of medicated serum on mushroom tyrosinase activity and melanogenesis were detected in vitro. Results： The aqueous extract and ethanol soluble extract of Yiqiqubai Compound could stimulate mushroom tyrosinase activity and melanogenesis compared with the normal group （P〈0.05）, and especially the aqueous extract was better. Conclusion： Yiqiqubai Compound with the up-regulation effect of tyrosinase activity and melanogenesis has important value to vitiligo treatment.