薯蓣皂苷元含药血清对IL-17和TNF-α诱导大鼠滑膜细胞株RSC-364 NF-κB p65、AP-1的影响

Effects of medicated serum with diosgenin on NF-κB p65 and AP-1 expression in rat synovial cell strain RSC-364 induced by IL-17 and TNF-α

目的:通过观察薯蓣皂苷元含药血清对白介素-17(IL-17)和肿瘤坏死因子-α(TNF-α)联合诱导的大鼠滑膜细胞株RSC-364核转录因子-κB(NF-κB)p65及活化蛋白-1(AP-1)表达的影响,探讨薯蓣皂苷元抑制类风湿关节炎(RA)血管新生可能的信号转导通路作用机制。方法:实验分为空白对照组、RA细胞模型组、薯蓣皂苷元含药血清组与雷公藤含药血清组,应用TransAM^(TM) NF-κB p65活性检测试剂盒检测NF-κB p65的DNA结合活性,应用EMSA方法检测AP-1的DNA结合活性。结果:与RA细胞模型组相比,薯蓣皂苷元含药血清组的NF-κB p65DNA结合活性显著降低(P<0.01),薯蓣皂苷元含药血清组AP-1的DNA结合活性降低(P<0.01)。结论:薯蓣皂苷元含药血清可以抑制IL-17和TNF-α联合诱导的大鼠滑膜细胞株RSC-364细胞模型NF-κB p65、AP-1的DNA结合活性,从而起到抑制RA血管新生的作用。

Objective： To observe the effects of medicated serum with diosgenin on NF＊rB p65 and AP-1 expression in rat synovial cell strain RSC-364 induced by IL-17 and TNF-a, and investigate the underlying mechanism about signal lransduction pathways of diosgenin on antiangiogenesis in treating rheumatoid arthritis＇ （RA）. Methods： The cells were divided into 4 groups： blank control group, RA model group, diosgenin medicated serum group and tripterygium medicated serum group. The NF-xB p65 expression in rat synovial cell strain RSC-364 in each group was detected by TransAMTM, the AP-1 expression in rat synovial cell strain RSC-364 in each group was detected by EMSA. Results： The NF-xB p65 activity in diosgenin medicated serum group was remarkably lower than that RA model group （P〈0.01）. The AP-1 DNA-binding activity in nuclear extract of diosgenin medicated serum group were remarkably lower than that RA model group （P〈0.01）. Conclusion： Diosgenin could remarkably lower NF- xB p65 and AP-1 DNA-binding activity in rat synovial cell strain RSC-364 induced by IL-17 and TNF-a, thereby to restrain angiogenesis of rheumatoid arthritis.