Lactobacillus casei胞外多糖对体外培养的巨噬细胞分泌TNF-α、NO及IL-10的影响

Effects of exopolysaccharide from Lactobacillus casei on TNF-α,NO and IL-10 secretion of mouse peritoneal macrophage cells in vitro

目的:探究干酪乳杆菌胞外多糖(EPS)对体外培养的小鼠腹腔来源巨噬细胞分泌炎症相关因子的影响。方法:采用倒置显微镜,观察干酪乳杆菌EPS对腹腔来源巨噬细胞形态的影响;采用双抗体夹心ELISA和Griess法,分析干酪乳杆菌EPS对腹腔来源巨噬细胞分泌促炎症因子TNF-α、NO及抗炎症因子IL-10的变化。结果:EPS能够抑制由脂多糖导致的巨噬细胞形态的变化,与空白组相比,350、400和450μg/ml EPS均能提高TNF-α、NO及IL-10的分泌量。脂多糖(Lipopolysac-charide,LPS)能诱导TNF-α和NO的分泌,抑制IL-10的分泌。350、400和450μg/ml EPS能抑制由10μg/ml脂多糖诱导的TNF-α和NO的分泌,促进LPS抑制的巨噬细胞分泌IL-10,且呈剂量依赖关系。结论:干酪乳杆菌EPS能双向调节体外培养的腹腔巨噬细胞分泌炎症相关因子功能。

Objective：To explore the effect of Lactobacillus casei exopolysaccharide（EPS） on inflammation-related factors secretion of macrophages in the mouse peritoneal in vitro.Methods： Using an inverted phase microscop,we observed the morphology of macrophages in the mouse peritoneal exposed to Lactobacillus casei EPS.The level of mouse TNF-α and IL-10 in culture supernatant was determined by sandwich enzyme-linked immunosorbent assay.The level of NO was detected by NO analyzing Kit.Results： EPS decreased the LPS-induced morphological changes and their secretion of the proinflammatory factor TNF-α,NO and anti-inflammatory factor IL-10 were increasing after treatment with 350 μg/ml,400 μg/ml and 450 μg/ml EPS compared with untreated controls.Lipopolysaccharides（LPS） induced the secretion of TNF-α,NO and suppressed the secretion of IL-10 by peritoneal macrophages.350 μg/ml,400 μg/ml and 450 μg/ml EPS suppressed 10 μg/ml LPS-induced release of TNF-α and NO by peritoneal macrophages and promoted 10 μg/ml LPS-induced suppression of IL-10,with dose-dependent effect.Conclusion： The EPS of Lactobacillus casei could influence inflammation-related factors secretion function of peritoneal macrophages by two-way regulation.