摘要
目的:探讨抗髓过氧化物酶抗体(AMPA)及抗乳铁蛋白抗体(ALA)对自身免疫性肝炎(AIH)检测的临床意义及评估。方法:对63例AIH(53例AIH-Ⅰ和10例AIH-Ⅱ)、206例非AIH患者和50例健康体检者均采用ELISA法检测AM-PA和ALA;间接免疫荧光法(IIF)检测抗核抗体(ANA)、抗平滑肌抗体(ASMA)和抗中性粒细胞胞质抗体(ANCA),观察临床评价指标,并对结果进行回顾性分析。结果:63例AIH和206例非AIH检测AMPA阳性率分别是61.9%(39/63)和3.40%(7/206),经χ2检验,P<0.01;AIH组中AIH-Ⅰ组阳性率73.6%(39/53)。63例AIH患者检测ALA阳性12例(19.0%),AIH-Ⅰ中阳性11例(20.8%)。AIH自身抗体临床评价指标显示ALA敏感性最低为19.05%,但其特异性最高为99.6%,AM-PA特异性为97.27%。结论:AMPA、ALA与AIH的发生发展存在相关性,两者联合其他自身抗体检测对AIH的诊断及其亚型的鉴别有重要意义。
Objective:To explore the significance and clinical value of anti-myeloperoxidase antibody(AMPA) and anti-lactoferrin antibody(ALA) detection in diagnosis of autoimmune hepatitis(AIH).Methods: A total of 63 AIH patients,including 53 AIH-Ⅰ and 10 AIH-Ⅱ,206 non-AIH patients and 50 healthy volunteers were included in this subject.Enzyme-linked immunosorbent assay(ELISA) was utilized to detect AMPA and ALA while indirect immunofluorescence(IIF) was employed to detect anti-nuclear antibodies(ANA),anti-smooth muscle antibodies(ASMA) and anti-neutrophil cytoplasmic antibodies(ANCA).Investigate the clinical characteristics and analyze the results respectively.Results: The positive rates of AMPA were 61.9%(39/63) in AIH group compared with 3.40%(7/206) in non-AIH group(P〈0.01) and 73.6%(39/53) in AIH-Ⅰ group.The positive rates of ALA were 19.0%(12/63) in AIH group and 20.8%(11/53) in AIH-Ⅰ group.The clinical indexes of AIH associated autoantibodies indicated that the ALA had the lowest sensitivity(19.05%),but with the highest specificity(99.6%).The specificity of AMPA was 97.27%.Conclusion: AMPA and ALA may play a role in the development of AIH,the combined detection of AMPA and ALA would be helpful in the diagnosis of AIH and AIH subtypes.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2013年第8期839-843,847,共6页
Chinese Journal of Immunology
基金
南昌市科技局基金(2011-ZCSF-YLWSYRKJK-066)资助项目
关键词
抗髓过氧化物酶抗体
抗乳铁蛋白抗体
自身免疫性肝炎
抗核抗体
抗平滑肌抗体
抗中性粒细胞胞质抗体
Anti-myeloperoxidase antibody(AMPF)
Anti-lactoferrin antibodies(ALA)
Autoimmune hepatitis(AIH)
Anti-nuclear antibody(ANA)
Anti-smooth muscle antibodies(ASMA)
Anti-neutrophil cytoplasmic antibody(ANCA)