小鼠胚胎发育过程中N-Cadherin异常表达对大脑皮层神经元迁移的影响

The effect of N-Cadherin misexpression on neuron migration during mouse cerebral cortex development

目的:探究N-Cadherin异常表达对小鼠大脑皮层发育过程中神经元迁移的影响。方法:通过小鼠活体子宫电转基因技术和RNA干扰技术相结合,在小鼠胚胎大脑皮层发育过程中实现对N-Cadherin的超表达和抑制表达,荧光免疫组化检测超表达结果,用GFP示踪N-Cadherin超表达和抑制表达后神经元在大脑皮层发育过程中的迁移特征。结果:与对照组相比,N-Cadherin超表达后有更多的GFP阳性神经元迁移到皮层的Ⅱ-Ⅳ层;N-Cadherin被抑制后大量GFP阳性神经元停滞在SVZ区。结论:N-Cadherin在小鼠大脑皮层发育过程中对神经元的迁移具有影响作用。

Objective：To explore the effect of N-Cadherin misexpression on neuron migration during cerebral cortex development of mouse.Methods： The overexpression and suppression of N-Cadherin were investigated with the methods of in utero electroporation and RNAi during mouse cortical development.After N-Cadherin overexpression or suppression with RNAi,the GFP was used to trace the migrated neurons in the developing cerebral cortex.Immunofluorescence histochemistry was carried out to analyze the overexpression results.Results： Compared to the controls,there were more GFP positive neurons moving to cortical layerⅡ-Ⅳ after N-Cadherin overexpression.However,a large number of GFP postive neurons were delayed in SVZ area without normal migration after the suppression of N-Cadherin.Conclusion： N-Cadherin can affect the migration of neurons during mouse cerebral cortex development.