固相萃取柱-HPLC测定植物油脂中苯并芘含量

Determination of Benzo(a)pyrene in Vegetable Oil by HPLC

采用固相萃取柱-HPLC法，利用定性（保留时间）、定量（峰面积）和分离度等指标，优化样品前处理方法和色谱条件，测定植物油脂中苯并芘的含量。色谱条件是：固相萃取柱，Agilent Eclipse PAH—C18色谱柱，流动相V（乙腈）：y（水）=88：12，流速为1．0ml／min，荧光检测器，正己烷为溶剂，进样量40μL，外标法定量。结果表明：该方法检出限为0．15μg／kg，加标回收率为89．4％-100．1％，变异系数为1．3％～2．4％；同时，样品前处理简单，目标峰保留时间短。

By the solid phase extraction column-HPLC in determinating benzopyrene of vegetable oils.Using of the qualitative （retention time） ,quantitative （peak area） and the separation index,determine the sample pretreatment method and the chromato- graphicconditions.Theresuhsshowthat：withthecolumnof Agilent Eclipse PAH-C18（5.0μm,4.6mm×250mm）,themobilephase of acetonitrile：water （88：12） ,the flow of 1.0 ml/min,and the injection volume of 40μL.the residual of benzopyrene in vegetable oil was determined. The limit of detection was 0.15μg/kg, overall recoveries were 89.4%- 100.1%, and RS D was 1.3 %-2.4%.