血清VEGF、TGF-β1及内皮抑素在非小细胞肺癌中的表达及相关性研究

Research on serum expression levels of VEGF,TGF-β1 and endostatin in patients with NSCLC and their correlation

目的检测血清中血管内皮生长因子(VEGF)、转化生长因子β1(TGF-β1)及内皮抑素在非小细胞肺癌(NSCLC)中的表达情况,探讨三者与NSCLC临床病理特征间的关系及三者彼此之间的关系。方法用双抗体夹心酶联免疫吸附试验(ELISA)检测63例NSCLC患者术前VEGF、TGF-β1及血清内皮抑素的水平,并与26例肺良性病变患者和28名健康对照者做比较。结果 NSCLC患者血清VEGF、TGF-β1及内皮抑素水平显著高于肺良性病变患者和健康对照者(P均<0.01)。血清VEGF、TGF-β1及内皮抑素水平与NSCLC细胞分化程度、TNM分期、有无远处转移密切相关(P<0.05);与组织学类型、有无淋巴结转移、年龄、性别和吸烟与否无关(P>0.05)。NSCLC患者血清TGF-β1与VEGF水平呈正相关(r=0.456,P<0.01),VEGF及TGF-β1与内皮抑素水平呈负相关(r=-0.363、-0.329,P均<0.05)。结论 VEGF、TGF-β1及内皮抑素共同参与了NSCLC血管生成,与细胞分化不良有密切关系,在NSCLC的发生、发展、转移中起重要作用,可能是预测肺癌恶性行为的有用指标。

Objective To determine the serum expression levels of vascular endothelial growth factor （ VEGF）, transforming growth factor-beta 1 （TCF-β1） and endostatin in patients with non-small cell lung cancer（NSCLC） , and to investigate the relationship of them with NSCLC clinical and pathological character and the relationship of them to each other. Methods Serum levels of VEGF,TGF-β1 and endostatin were determined by double-antibody sandwich enzyme- linked immunosorbent assay （ELISA） in 63 patients with NSCLC before surgery, and the resuhs were compared with those in 26 patients with benign pulmonary lesion and 28 healthy controls. Results The serum levels of VEGF, TGF-β1 and endostatin in NSCLC group were significantly higher than those in benign pulmonary lesion group and healthy control group （ P 〈0.01 ）. The serum levels of VEGF, TGF-β1 and endostatin were closely correlated with cell differentiation, tumor node metastasis （TNM） stage and metastasis （ P 〈0.05 ）, while they were not correlated with histological type, lymph node metastasis, age, sex and smoking or not（ P 〉 0.05 ）. The serum levels of TGF-131 and VEGF were positively correlated in NSCLC group （ r = 0. 456, P 〈 0. 01 ） , and were negatively correlated between endostatin and VEGF and between endostatin and TGF-131 （ r = - 0. 363 and - 0. 329, P 〈 0.05 ）. Conclusions VEGF, TGF-~I and endostatin are closely related with NSCLC angiogenesis and poor cell differentiation. For the formation, development and transfer of NSCLC, VEGF, TGF-β1 and endostatin may be useful indicators for the orediction of lung cancer.