变构菌素类似物的分离鉴定及其生物合成机制

The Isolation and Identification of Tautomycetin Analogue and Its Biosynthetic Mechanism

利用正相色谱、反相色谱法对灰产色链霉菌的发酵副产物进行了分离,采用波谱学方法进行了化合物的结构鉴定;利用生物转化方法对其生物合成途径进行了研究;采用MTT法检测了化合物对HCT-15细胞的抗肿瘤活性.结果表明:从灰产色链霉菌发酵产物中得到了变构菌素的结构类似物A1,A1为全新结构的天然产物.对比变构菌素,A1对HCT15的细胞毒性较差(IC50为70μm),且A1来自于变构菌素的异常生物合成.

The structural analogue from streptomyces griseochromogenes fermentation broth was isolated and identified, and its biosynthesis pathway was demonstrated and proposed. A1 was isolated by use of the normal phase and reverse phase chromatography methods. Its structure was elucidated from spectroscopic analysis. The biotransformation experiment was used to confirm its biosynthetic pathway, and its anti-tumor activity against HCT 15 was analyzed with MTT method. A novel metabolite is isolated and characterized as A1, a tautomycetin amide analogue, which has a moderate anti-tumor activity（IC50= 70μm）compared to tautomycetin. As a major by-product, A1 derives from the abnormal biosynthesis of tautomycetin production.