微生物来源的透明质酸酶的分离纯化与性质研究

Study on Purification and Character of Hyaluronidase from Microorganism

从噬尼古丁节杆菌的发酵液中初步分离纯化出透明质酸酶,并进行酶学性质研究。使用6000分子量的中空纤维膜将粗酶液超滤浓缩脱盐,冷冻干燥成粉末状,得到透明质酸酶;对粗酶液进行柱层析分离纯化,测定粗酶液和纯化酶的活性。该透明质酸酶的酶学性质研究表明,从噬尼古丁节杆菌的发酵液中分离得到一种新的透明质酸酶,其性质稳定、活性较高、受环境影响较小、易于分离纯化,为透明质酸酶的开发提供了重要的实验依据。

Extracellular hyaluronidase from culture filtrates was studied. And the condition of euhuro of Arthrobacter nicotinovorans was optimized. Hyaluronidase was purified to homogeneity by the ultra-filtration through the hollow fiber membrane of 6000 Da, and freeze - dried into a powder. A kind of Hyaluronidase was discovered, seperated and purified by using column chromatography. Its biochemical characteristics were stable and embraced high enzymatic activity. This enzyme was little affected by the environment and was easy to purify. This result provided an important basis for the exploitation of a Hyaluronidase.