生长抑素和胰岛淀粉样多肽在生后发育期家兔胃窦黏膜的定位和表达

Localization and expression of somatostatin and islet amyloid polypeptide in rabbit gastric antrum mucosa during postnatal development period

目的:观察家兔生后发育期间生长抑素(SS)和胰岛淀粉样多肽(IAPP)在胃窦黏膜中的定位和表达,探讨其与机体消化功能的关系。方法:生后5、15、25、35、60和90d家兔各10只,处死后取胃窦组织,常规石蜡包埋,制成连续切片,用免疫组织化学SABC法和图像分析法对SS、IAPP免疫反应阳性细胞的定位和表达进行分析。结果:不同发育阶段兔胃窦黏膜内均有SS和IAPP的表达。阳性产物呈棕黄色,位于细胞质内。家兔生后5和15d,SS阳性细胞分散存在于黏膜上皮内,染色较浅,35d开始逐渐增多,于60d时数量最多(P<0.05),90d时减少至35d水平;细胞平均灰度值于35d时降低,60d时达到最低(P<0.05)。IAPP阳性细胞多数定位于胃窦黏膜结缔组织中,家兔从生后5d开始IAPP阳性细胞数量逐渐增多,尤以60d增多明显(P<0.05);其平均灰度值于35d之后降低,低于5、15和25d组(P<0.05)。结论:SS和IAPP在家兔胃窦黏膜中的表达随生长发育而发生变化,二者可能以内分泌、旁分泌的方式参与生后机体的消化活动,并在胃黏膜的生长修复和自我保护中发挥重要作用。

Objective To study the localization and expressions of somatostatin（SS）and islet amyloid polypeptide（IAPP）in rabbit gastric antrum mucosa during the postnatal development period,and to explore the relationship between these hormones and digestive function.Methods Sixty rabbits were divided into 6groups according to their age：5d,15d,25d,35d,60d,and 90d;10rabbits in each group.The rabbits were excuted and their gastric antrum tissues were taken and embedded in routine paraffin,then the serial sections were made.Immunohistochemical SABC staining and image analysis were performed to detect the expression and localization of SS and IAPP immunoreactive positive cells.Results The expressions of SS and IAPP were found in the gastric antrum mucosa of rabbits at different postnatal development periods.In 5dand 15dgroups,a few SS positive cells were scattered into the mucosa epithelium and the staining was weaker.From the 35th day,the number of SS positive cells was ascended and obviously reached a peak in 60dgroup（P0.05）,and the number of SS positive cells in 90dgroup was similar with that in 35dgroup.The mean grey value of SS positive cells was decreased from the 35th day and reached the lowest in 60dgroup（P0.05）.The positive IAPP positive cells were predominantly located in the connective tissue of gastric antrum mucosa,occasionally in the gastric mucosal epithelium.The number of IAPP positive cells was gradually increased from the 5th day,especially in 60dgroup（P0.05）.The mean grey value of IAPP positive cells was decreased from the 35th day（P0.05）.Conclusion The expressions of SS and IAPP in gastric antrum mucosa of rabbits change with the growth,which suggests that both of them should participate in the process of the digestive function by endocrine and paracrine ways.They play an important role in the growth repair and self-protection of the gastric mucosa.