宫颈癌患者血清和癌组织中NF-κB及TNF-α蛋白的表达及其意义

Expressions of NF-κB and TNF-αprotein in serum and carcinoma tissue of patients with cervical cancer

目的:探讨不同宫颈病变患者血清和宫颈组织中核因子(NF-κB)及肿瘤坏死因子α(TNF-α)蛋白的表达,阐明其生物学意义和早期联合诊断的价值。方法:对100例有宫颈癌病变高危因素的患者(宫颈炎37例、宫颈内瘤变39例和宫颈癌24例)抽取静脉血同时行阴道镜检。采用ELISA方法检测血清及镜检宫颈组织中NF-κB和TNF-α蛋白的含量;采用RT-PCR法检测宫颈组织中NF-κB及TNF-α的mRNA表达水平;采用免疫组织化学方法检测宫颈组织中NF-κB及TNF-α的蛋白表达;结合临床病理特点进行分析。结果:宫颈内瘤变及宫颈癌患者血清及镜检组织中NF-κB和TNF-α含量明显高于宫颈炎患者(P<0.01),宫颈癌患者其含量明显高于宫颈内瘤变患者(P<0.01);宫颈内瘤变和宫颈癌患者宫颈组织中NF-κB和TNF-αmRNA及其蛋白表达水平明显高于宫颈炎患者(P<0.01),且宫颈癌患者宫颈组织中其表达水平明显高于宫颈内瘤变患者(P<0.01)。宫颈炎、宫颈内瘤变及宫颈癌患者宫颈组织中NF-κB与TNF-α含量呈正相关关系(r=0.69,P<0.05)。结论:NF-κB和TNF-α均可独立作为早期诊断宫颈癌前病变及宫颈癌的标记物,二者的正相关性使得联合检测可提高宫颈癌的早期诊断率。

Objective To explore the expressions of nuclear factor（NF-κB）and tumor necrosis factor（TNF-α） protein in serum and carcinoma tissue of the patients with cervical cancer,and to clarify their biological significance and the values in early combined diagnosis.Methods The blood samples were collected and colposcope was performed in 100patients with high-risk factors of cervical lesion（37cases of cervicitis,39cases of cervical intraepithelial neoplasia,24cases of cervical cancer）.The ELISA method was used to detect the contents of NF-κB and TNF-αprotein in the serum and cervix tissue.The expression levels of NF-κB and TNF-αmRNA in cervix tissue were determined with RT-PCR assay.The immunohistochemical method was used to detect the expressions of NF-κB and TNF-αprotein in cervix tissue;the relationship with clinical and pathological features was analyzed.Results The contents of NF-κB and TNF-αprotein in serum and cervix tissue in the patients with cervical intraepithelial neoplasia and cervical cancer were significantly higher than those in the patients with cervicitis（P 0.01）.The contents of NF-κB and TNF-αprotein in the patients with cervical cancer were significantly higher than those in the patients with cervical intraepithelial neoplasia（P0.01）.The NF-κB and TNF-αmRNA expression levels and protein levels in the cervix tissue of the patients with cervicitis were significantly lower than those of the patients with cervical neoplasia and cervical cancer（P0.01）,and the expression levels NF-κB and TNF-αmRNA and NF-κB and TNF-α protein in cervical tissue of the patients with cervical intraepithelial neoplasia were significantly lower than those of the patients with cervical cancer（P0.01）.The expressions of mRNA and protein of NF-κB and TNF-αshowed positive correlation between the patients with cervicitis,cervical intraepithelial neoplasia and cervical cancer（r=0.69,P=0.037）.Conclusion NF-κB and TNF-αcan be independent as markers for early diagnosis of cervical precancerous lesions and cervical cancer,and the positive correlation between them can make the combined detection to improve the early diagnosis rate of cervical cancer.