响应面法优化连翘中桦木酸超声辅助提取工艺

Optimization of Ultrasound-Assisted Extraction Technology for Betulinic Acid from Forsythiae Fructus by Response Surface Methodology

目的:应用响应面法优化超声辅助提取连翘中桦木酸的工艺条件。方法:在单因素试验基础上,采用Box-Behnken试验设计和响应面分析法考察乙醇体积分数、提取温度、液固比对桦木酸提取率的影响。HPLC测定桦木酸含量,Thermo Hypersil GOLD AQ-C18色谱柱(4.6 mm×250 mm,5μm),流动相乙腈-水(9∶1),流速1.2 mL·min-1,柱温25℃,进样量20μL,检测波长210 nm。结果:最佳超声提取工艺为乙醇体积分数80%,提取温度37℃,液固比32 mL.g-1,桦木酸提取率(4.28±0.05)mg·g-1,与模型预测值(4.30 mg·g-1)基本一致。结论:该工艺合理、可靠,有效成分提取率高。

Objective：To optimize ultrasonic-assisted extraction technology of betulinic acid from Forsythiae Fructus using response surface methodology.Method：On the basis of single factor tests,Box-Behnken experimental design and response surface methodology was adopted to optimize extraction conditions with the concentration of ethanol,extraction temperature and solid-liquid ratio as factors.HPLC was used to determine the content of betulinic acid from Forsythiae Fructus,chromatographic conditions were as follows：Thermo Hypersil GOLD AQ-C18 column（4.6 mm × 250 mm,5 μm）,acetonitrile-water（9：1） as mobile phase,flow rate of 1.2 mL·min-1,injection volume 20 μL,detection wavelength at 210 nm.Result：Optimal ultrasound-assisted extraction technology of betulinic acid from Forsythiae Fructus was as following：the volume fraction of ethanol 80%,extraction temperature 37 ℃,liquid-solid ratio 32 mL.g-1.The actual value of betulinic acid yield was（4.28 ± 0.05） mg·g-1,which was consistent with the predicted value of 4.30 mg·g-1.Conclusion：This optimized extraction process was reasonable and reliable with high yield of effective components.