正交试验法优选健胃清肠合剂的水提醇沉工艺

Optimization of Water Extraction and Alcohol Precipitation Technology for Jianwei Qingchang Mixtures by Orthogonal Test

目的:优选健胃清肠合剂的水提醇沉工艺。方法:以大黄酸、大黄素、大黄酚总含量和干膏得率的综合评分为指标,采用正交试验考察加水量、提取次数、提取时间对水提取工艺的影响;以大黄酸、大黄素、大黄酚总含量为指标,通过正交试验考察醇沉浓度和浓缩液相对密度对醇沉工艺的影响。结果:最佳提取、醇沉工艺为加10倍量水提取2次,每次3 h,合并提取液,浓缩至相对密度1.05 g·mL-1,加乙醇至乙醇体积分数60%除去沉淀。结论:优选的提取、醇沉工艺稳定可行,为健胃清肠合剂规范化生产提供参考。

Objective：To optimize water extraction and alcohol precipitation technology of Jianwei Qingchang mixtures.Method：With composite score of the total content of rhein,emodin and rhubarb phenol,dry extract yield as index,effects of the amount of water,extraction times and time on extraction technology was investigated by orthogonal test;With the total content of rhein,emodin and rhubarb phenol as index,orthogonal test was adopted to investigate effects of the concentration of alcohol precipitation and relative density of concentrate on alcohol precipitation technology.Result：Optimum water extraction and alcohol precipitation technology was as following：extracted 2 times with 10 folds the amount of water,3 h each time,extracts were combined and concentrated to a relative density of 1.05 g.mL-1,added 95% ethanol to precipitate the volume fraction of ethanol with 60%.Conclusion：Optimized water extraction and alcohol precipitation technology was stable and feasible,it could provide a reference for standardized production of Jianwei Qingchang mixtures.