苦参药材中氧化苦参碱和苦参总黄酮含量比较

Comparative Study on Contents of Oxymatrine and General Flavone in Sophora Flavescens from Different Habitats

目的:测定不同产地、来源苦参药材中氧化苦参碱和苦参总黄酮的含量。方法:采用高效液相法检测苦参中氧化苦参碱的含量,采用紫外分光光度法检测苦参中苦参总黄酮的含量。结果:比较了10批不同产地、来源苦参药材中氧化苦参碱和苦参总黄酮的含量,其中河南产苦参(批号110613)氧化苦参碱含量最高,达19.53 mg·g-1,广西产苦参(批号110930)中苦参总黄酮含量明显高于其他产地的苦参,为14.44 mg·g-1。结论:不同产地、来源苦参药材中有效成分的含量差异较大,在临床用药时应引起重视,在含苦参的复方制剂生产中应建立药材检测的可控标准。

Objective：To study the content of oxymatrine and general flavone in Sophora flavescens from different habitats.Method：HPLC and UVspectrophotometry method were applied to detected the content of oxymatrine and general flavone in S.flavescens.Result：S.flavescens from different habitats were detected.Oxymatrin content in S.flavescens from Henan Province（Batch No：110613） was the highest,up to 19.53 mg·g-1,and general flavone content in S.flavescens from Guangxi Province（Batch No：110930） was 14.44 mg·g-1,significantly higher than the others.Conclusion：Oxymatrine and general flavone content differ widely in S.flavescens from different habitats,and it is necessary to establish quality control standard for compound preparations of S.flavescens.