HPLC指纹图谱法评价市售胖大海的质量一致性

Quality Coherence Evaluation of the Seeds of Sterculia lychnophora Commercially Available in China Based on HPLC Fingerprint

目的:建立胖大海高效液相色谱指纹图谱分析方法,比较不同地区市售胖大海质量的一致性,为其质量评价提供参考。方法:通过对提取条件和色谱条件进行考察,构建高效液相色谱指纹图谱法并用其测定购自国内不同地区的市售胖大海样品的指纹图谱,采用相似度分析软件对指纹图谱进行分析比较。结果:确定了胖大海指纹图谱的提取条件和高效液相色谱条件,发现了胖大海的20个共有特征峰,8份市售胖大海样品的相似度为0.942～0.984。结论:建立了胖大海的高效液相色谱指纹图谱分析方法,通过该方法证明中国不同地区的市售胖大海的质量比较一致。

Objective：To establish high performance liquid chromatographic fingerprint of the seeds of Sterculia lychnophora and compare the quality coherence of this drug commercially available in China in order to provide a reference for its quality evaluation.Method：The fingerprint analysis procedure of these seeds was developed by investigating the extraction and chromatographic conditions,the fingerprints of 8 batches of the seeds purchased from various places of China were determined with this procedure,and the data were analyzed with Chromatographic Fingerprint Similarity Evaluation System 2004A Version provided by Chinese Pharmacopoeia Commission.Result：The extraction and chromatographic conditions for HPLC fingerprint of the seeds of S.lychnophora were established,20 common peaks of this drug were identified,and similarities of these 8 batches were determined to be between 0.942 and 0.984.Conclusion：The HPLC fingerprint analysis procedure of the seeds of S.lychnophora has been successfully established for the first time.The quality of the seeds of S.lychnophora commercially available in China was demonstrated to be comparable by the established procedure.