UPLC法测定半夏提取物中尿苷、鸟苷和腺苷的含量

Determination of Content of Uridine,Guanosine and Adenosine in Pinellia ternata by UPLC

目的:建立以超高效液相色谱(UPLC)法测定半夏提取物中尿苷、鸟苷和腺苷含量的方法。方法:色谱柱ACQUITY UPLC BEH Cl8(2.1 mm×50 mm,1.7μm),流动相水-甲醇不同比例梯度洗脱,柱温30℃,流速0.5 mL·min-1,检测波长254 nm。结果:尿苷在2.033～20.33 mg·L-1线性关系良好,回归方程为Y=16 788X-3 556(r=0.999 9),平均回收率98.65%,RSD 2.12%;鸟苷在1.098～10.98 mg·L-1线性关系良好,回归方程为Y=25 378X-325(r=0.999 9),平均回收率98.21%,RSD 2.32%;腺苷在0.201 2～2.012 mg·L-1线性关系良好,回归方程为Y=30 800X-59(r=0.999 9);平均回收率98.54%,RSD 1.91%。结论:该方法准确、快速、可靠,能有效地测定半夏提取物中尿苷、鸟苷和腺苷的含量。

Objective：To establish an ultra performance liquid chromatography（UPLC） method for detecting content of uridine,guanosine and adenosine in Pinellia ternata.Method：ACQUITY UPLC BEH Cl8（2.1 mm ×50 mm,1.7 μm） was used,and the mobile phase was composed of methanol and water by gradient elution mode,column temperature was 3 0 ℃,at a flow rate of 0.5 mL·min-1 and detection wavelength of 254 nm.Result：The linear range of uridine was 2.033-20.33 mg·L-1.The regression equation was as follows：Y = 16 788X-3 556（r = 0.999 9）.The linear range of guanosine was 1.098-10.98 mg·L-1.The regression equation was as follows：Y = 25 378X-325（r = 0.999 9）.The linear range of adenosine was 0.201 2-2.012 mg·L-1.The regression equation was as follows：Y = 30 800X-59（r = 0.999 9）.The average recovery was 98.65%（RSD 2.12%）,98.21%（RSD 2.38%） and 98.54%（RSD 1.91%） respectively.Conclusion：The method is accurate,rapid and reliable and can be used to determine the content of uridine,guanosine and adenosine in P.ternata.