HPLC测定定心藤中汉防己碱的含量

Determination of Tetrandrine in Mappianthus iodoides by HPLC

目的:建立定心藤中汉防己碱的含量测定方法。方法:采用高效液相色谱法,VP-ODS C18色谱柱(4.6 mm×250 mm,5μm),流动相乙腈-0.3%三乙醇胺溶液(磷酸调pH 2.0)(45∶55),流速1.0 mL·min-1,检测波长280 nm。结果:定心藤中的汉防己碱含量测定方法在0.040 4～0.363 6μg进样量与峰面积的线性关系良好(r=0.999 9),平均回收率99.02%,RSD 1.67%。结论:所建立的方法准确、灵敏、重复性好,可用于定心藤的质量控制。

Objective：To establish the determination method of tetrandrine content in Mappianthus iodoides.Method：VP-ODS Cl8（4.6 mm × 250 mm,5 μm） colmnn was used,the mobile phase was acetonitrile-0.3% triethanolamine（with H3PO4 to adjust pH 2.0）（45 ：55）,the flow rate was 1.0 mL·min-1.The detection wavelength was sest at 280 nm.Result：The linear relation of hyperin was good in the range from 0.040 4 to 0.363 6 μg.The average recovery was 99.02%,RSD was 1.67%.Conclusion：The method established is accurate,sensitive and good in reproducibility.It can be applied to the quality control of Mappianthus iodoides.