承德产山楂叶中总黄酮和5种黄酮类成分含量的动态分析

Dynamic Analysis of Total Flavonoids and Five Flavonoid Components in Hawthorn Leaves from Chengde

目的:对不同月份承德产山楂叶中总黄酮以及牡荆素葡萄糖苷、牡荆素鼠李糖苷、牡荆素、芦丁、金丝桃苷5种黄酮类成分进行含量测定及动态分析,以确定山楂叶的最佳采收期。方法:采用紫外分光光度法测定不同月份山楂叶中总黄酮含量,同时用高效液相色谱法测定5种黄酮类成分含量,并分析其不同月份含量的动态变化。Agilent ZORBAX SB-C18色谱柱(4.6 mm×250 mm,5μm),流动相0.1%甲酸水溶液-乙腈-四氢呋喃梯度洗脱,流速1.0 mL·min-1,检测波长340 nm,柱温30℃,进样量10μL。结果:山楂叶总黄酮及牡荆素葡萄糖苷、牡荆素鼠李糖苷、金丝桃苷含量6月份最高,牡荆素、芦丁含量7月份最高。结论:若以提取山楂叶中黄酮类成分为目的,建议6月份采集。

Objective：To determine the content and analyze the dynamic changes of the total flavonoids and five flavonoid components of vitexin-glucoside,vitexin-rhamnoside,vitexin,rutin,hyperoside of different months in hawthorn leaves from Chengde,and then determine the optimal harvest time.Method：The content of the total flavonoids in hawthorn leaves was determined by UV spectrophotometry,and the five flavonoid components were determined by high performance liquid chromatography（HPLC） to analyze the dynamic changes of the content in different months.An Agilent ZORBAX SB-C18 column（4.6 mm × 250 mm,5 μm） was used with the mobile phase of 0.1% formic acid aqueous solution-acetonitrile-tetrahydrofuran at 30 ℃.The flow rate was 1.0 mL·min-1.The detection wavelength was set at 340 nm.Injection volume was 10 μL.Result：The content of the total flavonoids and vitexin-rhamnoside,vitexin-glucoside,hyperoside in hawthorn leaves were the highest in June.The content of vitexin and rutin was the highest in July.Conclusion：It is suggested that the best harvest time of hawthorn leaves for extracting flavonoid components is in June.