川楝子提取物在大鼠肠外翻试验中吸收特性考察

Absorption Characteristics Investigation of Extract from Toosendan Fructus in Rat Everted Gut Test

目的:采用肠外翻模型观察川楝子提取物在大鼠十二指肠、空肠、回肠及结肠段的吸收特征。方法:采用大鼠肠外翻模型,通过HPLC检测肠吸收液中川楝素(TSN)含量,计算吸收速率参数,以分析川楝素在肠道不同部位的吸收特征。结果:不同质量浓度的TSN在各肠段均为线性吸收,R2均>0.95,符合零级吸收;TSN的吸收速率常数均随川楝子提取物质量浓度的增加而显著增加,表现为被动吸收。川楝素在十二指肠、空肠、回肠吸收较好,且无显著性差异,但在结肠处吸收较差。结论:TSN在肠道不同部位吸收均符合零级吸收速率,吸收形式可能为被动吸收。

Objective：To observe absorption characteristics of extract from Toosendan Fructus in duodenum,jejunum,ileum and colon segment by rat everted gut model.Method：Toosendanin（TSN） which was representative compositions of extract from Toosendan Fructus in rat everted gut test was detected by HPLC,and absorption rate parameter to describe absorption characteristics of TSN in different parts of intestine was calculated.Result：Absorption of TSN with different concentration was linearity in different intestines,square of coefficient correlation all exceed 0.95,which consistent with zero order absorption.K of TSN significantly increased along with raised the concentration of extract from Toosendan Fructus,indicated passive absorption.Absorption of TSN in duodenum,jejunum,ileum was quick with no significant differences,but absorption of TSN in colon was slow.Conclusion：Absorption of TSN at different sections of intestine conformed to zero order model with a pattern of passive absorption.