卷柏中穗花杉双黄酮降血糖作用

Anti-diabetic Activity of Amentoflavone in Selaginella tamariscina in Diabetic Mice

目的:采用糖尿病小鼠模型,研究卷柏中穗花杉双黄酮(AMT)的降血糖活性,并进行量效关系考察。方法:雄性昆明种小鼠,一次性ip 180 mg·kg-1链脲佐菌素(STZ),建立糖尿病模型。以穗花杉双黄酮30,60,120,240,480 mg·kg-1ig给药治疗2周。给药期间测量小鼠摄食量、饮水量;给药2周后,检测空腹血糖(FBG),口服葡萄糖耐量(OGTT),血清胰岛素,观察胰腺HE染色切片,初步判断穗花杉双黄酮有效剂量。结果:穗花杉双黄酮30,60,120,240,480 mg·kg-1均能不同程度降低糖尿病小鼠饮水量、摄食量,FBG,改善OGTT,升高胰岛素水平;对胰岛组织还有一定修复作用。结论:穗花杉双黄酮可改善糖尿病小鼠血糖紊乱,调节胰岛素分泌,修复胰岛组织,最佳剂量为60 mg·kg-1。

Objective：To study the anti-diabetic activity of amentoflavone（AMT） in diabetic mice,and to lay the foundation for the further research and development.Method：Male Kunming mice were injected intraperitoneally（ip） with streptozotocin（STZ,180 mg·kg-1） to induce diabetic model.Then,the diabetic mice were treated with AMT（30,60,120,240,480 mg·kg-1） for 2 weeks.During the administration,food and water intake was recorded.After administration for 2 weeks,the serum levels of fasting blood glucose（FBG）,oral glucose tolerance test（OGTT） and insulin were measured,meanwhile pathologic-morphological of pancreas was observed by HE staining,to authenticate the antidiabetic activity of AMT and to find out the optimum dose.Result：All the dose of AMT（30,60,120,240,480 mg·kg-1） could decrease the food and water intake and FBG,and ameliorate the OGTT.Meanwhile,amentoflavone could improve islet tissue repair function.Conclusion：AMT can ameliorate the glucose disorder,regulate insulin secretion and restore the pancreas.