糖代谢异常代谢综合征大鼠中医证型的以方测证法研究(Ⅰ)

Syndrome Identification of Abnormal Glucose Metabolism in Metabolic Syndrome Rat Based on Syndrome Detecting from Recipe Used

目的:用"以方测证法"探讨糖代谢异常的代谢综合征(MS)大鼠可能出现的中医证型。方法:在建立糖代谢异常的MS大鼠模型后,用随机对照表法按血糖高低随机分为MS组、A组、B组、C组、D组。用4个方剂:大柴胡汤(A组,9.4g·kg-1)、黄连温胆汤(B组,10.4 g·kg-1)、知柏地黄丸(C组,15.1 g·kg-1)、血府逐瘀汤(D组,7.8 g·kg-1),于造模12周后ig给药,持续3周。于12周和15周末,检测其体重、血压、空腹血糖、甘油三酯、高密度脂蛋白胆固醇(HDL-C)及胰岛素(FINS)水平。结果:第15周时,与模型组比较A～D组体重、血压、空腹血糖(FBG)均无显著差异,A,B,D组TG水平显著降低(P<0.05或P<0.01),B组HDL-C显著升高(P<0.05),A～D组FINS和胰岛素抵抗指数(HOMA-IR)显著降低(P<0.01)。结论:糖代谢异常的MS大鼠可能同时存在肝胃郁热证、痰热互结证、阴虚热盛证、血瘀脉络证4种证型。不是单独存在,而是病理因素相互夹杂,混合在一起所组成的复合证型,出现于糖代谢异常的MS大鼠中。

Objective：To explore TCM syndromes in abnormalglucose metabolism metabolic syndrome（MS） rats based on syndrome detecting from recipe used.Method：After the establishment of the MS rat model,according to blood sugar level,rats were randomly divided into MS group,group A,group B,group C,group D.Chaihu Tang,Huanglian Wendan Tang,Zhibai Dihuang Wan and Xuefu Zhuyu Tang were given.At week 12 and 15 the weight,blood pressure,fasting blood glucose（FBG）,triglycerides（TG）,high density lipoprotein（HDL-C） and insulin levels were measured.Result：Compared with the model group,in A,B,C,D group,body weight,blood pressureand FBG had no significant difference,in A,B and D groups TG level significantly decreased,and in B group HDL-C was significantly increased（P ＆lt; 0.05）,in A and D group fasting insulin（FINS） and homeostasis model assessment insulin resistance（HOMA-IR） were significantly lowered（P ＆lt;0.01）.Conclusion：Abnormal glucose metabolism MS rats may exist hepatogastric stagnant heat,phlegm each node certificate,Yin Heat Syndrome and blood stasis syndrome,and the four syndromes indicated mixed signs.