哈蟆油石油醚提取物的抗抑郁作用

Antidepressant Effects of Petroleum Ether Extracts from Ranae Oviductus

目的:探索哈蟆油石油醚部位(ROS)的抗抑郁作用及可能的机制。方法:雄性ICR小鼠,分为2批,每批均设空白对照组(生理盐水)和6个治疗组(分别给予阳性药氟西汀10 mg·kg-1和ROS不同剂量10,30,100,300,900 mg·kg-1),连续灌胃给药8 d。第1批动物在第7天给药后1 h和第8天给药后1 h,分别采用小鼠强迫游泳和小鼠悬尾2种"行为绝望"抑郁动物模型进行行为学观察;行为学观察结束后小鼠眼球取血,用放射免疫法检测小鼠血清皮质酮(CORT)水平。第2批动物在第8天给药后采用利血平拮抗模型,观察ROS对由利血平引起小鼠体温下降和运动不能的影响。结果:ROS 100,300,900 mg·kg-1均能不同程度地缩短小鼠强迫游泳和悬尾不动时间(P<0.05),能拮抗利血平致小鼠体温下降和运动不能(P<0.05),降低小鼠血清皮质酮水平(P<0.05)。结论:ROS具有抗抑郁作用,其机制可能与单胺类神经递质以及下丘脑-垂体-肾上腺(HPA)轴密切相关。

Objective：To study the antidepressant effects of petroleum ether extracts from Ranae Oviductus（ROS） and to investigate the related mechanisms.Method：Male ICR mice were divided into 2 batches,and every batch was randomly divided into control and six treatment groups.The control group was given normal saline and treatment groups were received ROS at dose of 10,30,100,300,900 mg·kg-1 respectively for 8 days.In the first batch,1 h after the 7 th day and 8 th day administration,forced swimming test（FST） and tail suspension test（TST） were conducted for evaluation of behavior.And then the levels of serum corticosterone in the mice were measured by radioimmunoassay method.In the second batch,we observed the effects of ROS on depressive mice through reserpine reversal test after the day of 8 administration.Result：The group of 100,300,900 mg·kg-1 ROS could significantly shorten mice immobility time in FST and TST（P ＆lt; 0.05）,and could remarkably inhibit the decrease of body temperature and motor deficits in reserpine-induced hypothermia and motor deficits test at 4th hour（P ＆lt; 0.05）.Moreover,the groups of 100,300,900 mg·kg-1 ROS could decrease the cortisol level in mice serum compared with control group（P ＆lt; 0.05）.Conclusion：Petroleum ether extracts from Ranae Oviductus possess antidepressant effects,and it may be related to monoamine neurotransmitters and hypothalamic-pituitary-adrenal（HPA） axis.