热性惊厥儿童血液IL-6、IL-10和MMP-9表达的研究

Expression of IL-6,IL-10 and MMP-9 in Children Serum with Febrile Seizures

目的热性惊厥是儿童痫性发作最常见的形式。炎性细胞因子可能导致热性惊厥的发展。本研究探讨白介素-6(IL-6)、白介素-10(IL-100和金属蛋白酶-9(MMP-9)等炎性细胞因子在小儿热性惊厥中是否被激活以及其表达与原发性癫痫中的不同。方法回顾性研究自2010年12月到2012年11月入院的相关病人资料。分热性惊厥组(N=43)、高热对照组(N=40)、原发性癫痫组(N=32)及正常儿童组(N=15)。在惊厥发生的24h内收集病人血液。酶联免疫吸附试验(ELISA)进行IL-6、IL-10和MMP-9等细胞因子水平测定。结果 IL-6、IL-10和MMP-9在热性惊厥组的表达均高于原发性癫痫组(p<0.05)和正常组(p<0.05),IL-6与MMP-9在热性惊厥组的表达高于高热对照组,IL-10的表达在热性惊厥组与高热对照组之间无明显差异(P>0.05)。结论 IL-6、IL-10和MMP-9在热性惊厥儿童表达明显升高;炎性因子可能参与热性惊厥的病理过程,且其参与机制可能不同于其在原发性癫痫病理过程中的作用。

Objective Febrile seizures are the most common form of childhood seizures. Inflammatory eytokines may promote the development of febrile seizures. This study aimed to explore the expression of inflammatory cytokines such as IL-6 ,IL-10 and MMP-9 in children serum with febrile seizures and to confirm whether the expression is different compared with idiopathic epilepsy. Methods We performed a prospec- tive study from December 2010 to November 2012. The children were divided into 4 groups, the febrile seizures group, febrile infection group, idiopathic epilepsy group and normal control group. Serums were collected within 24 hs after febrile seizures attacked. The expression level of IL-6 ,IL-10 and MMP-9 were detected by ELISA kits. Results The expression of IL-6 ,IL-1 and MMP-9 in the febrile seizures group were sig- nifieantly higher than the febrile seizures group （ p 〈 0. 05 ） or the idiopathic epilepsy group （ p 〈 0.05 ）. The expression of IL-6 and MMP-9 in the febrile seizures group was significantly higher than the febrile infection group （ p 〈 0. 05 ） but no significantly differences were found about the expression of IL-10（ P 〉0. 05 ）. Conclusions The expression of IL-6 ,IL-10and MMP-9 are significantly higher in children serum with fe- brile seizures. Inflammatory cytokines may take part in the pathogenesis of febrile seizures, and the pathogenesis involving inflammatory eyto- kines probably different with idiopathic epilepsy.