七氟烷预处理对急性缺氧小鼠脑氧化应激的影响

Effects of sevoflurane preconditioning on oxidative stress of brain in acute hypoxia mice

目的:观察七氟烷预处理对急性缺氧小鼠脑氧化应激的影响,并探讨其与预处理的时间、剂量的关系。方法:60只雄性C57BL/6J小鼠随机分为6组:对照组(Control)、急性缺氧组(H组,6.5%O_2,24 h)、2%Sevo 30 min+H组、2%Sevo 60 min+H组、4%Sevo 30 min+H组、Propofol+H组。按试剂盒说明检测脑匀浆丙二醛(MDA)含量及超氧化物岐化酶(SOD)、谷胱甘肽过氧化物酶(GPx)活性。结果:与H组比较,各七氟烷预处理组脑内MDA水平下降,并在2%Sevo 60 min+H组、4%Sevo 30 min+H组降低显著,Propofol+H组则无意义;GPx活性在各七氟烷预处理组有所升高,在4%Sevo 30 min+H组上升显著,Propofol+H组GPx活性下降,但不明显;与预计相反,各预处理使SOD活性显著降低。结论:七氟烷预处理减轻脑氧化应激而具有脑保护作用,并与时间、剂量相关。

Aim：To investigate the effect of Sevoflurane （Sevo） preconditioning on oxidative stress of brain in acute hypoxia mice, and the relationship with duration of adminstering drug and concentration. Methods： Sixty male C56BL/6J mice were randomly allocated to 6 groups ： Control, Hypoxia （ H, 6. 5% 02for 24 h） ,2% Sevo 30 rain ＋ H, 2% Sevo 60 min ＋ H, 4% Sevo 30 min ＋ H,Propofol ＋ H. According to the kit instruction steps, the brain was homogenized for the measurement of malondialdehyde （ MDA）, superoxide dismutase （ SOD）, glutathione peroxidase （GPx）. Results： Compared with H group, the brain levels of MDA reduced in all Sevo precondition groups, moreover,in the 2% Sevo 60 min ＋ H group and 4% Sevo 30 min ＋ H group, significantly reduced, however Propofol ＋ H group was not statistically signif- icant; the activity of GPx increased in three Sevo precondition groups, and in the 4% Sevo 30 min ＋ H group, this increased significantly, activity of GPx reduced in the Propofol ＋ H group, but has not significant ; the activity of SOD decreased in all precondition groups which was not influenced as expected. Conclusion：Sevo preconditioning protects the mouse brain against oxidative stress, and the protection is related to duration of adminstering drug and dose.