摘要
目的:观察七氟烷预处理对急性缺氧小鼠脑氧化应激的影响,并探讨其与预处理的时间、剂量的关系。方法:60只雄性C57BL/6J小鼠随机分为6组:对照组(Control)、急性缺氧组(H组,6.5%O_2,24 h)、2%Sevo 30 min+H组、2%Sevo 60 min+H组、4%Sevo 30 min+H组、Propofol+H组。按试剂盒说明检测脑匀浆丙二醛(MDA)含量及超氧化物岐化酶(SOD)、谷胱甘肽过氧化物酶(GPx)活性。结果:与H组比较,各七氟烷预处理组脑内MDA水平下降,并在2%Sevo 60 min+H组、4%Sevo 30 min+H组降低显著,Propofol+H组则无意义;GPx活性在各七氟烷预处理组有所升高,在4%Sevo 30 min+H组上升显著,Propofol+H组GPx活性下降,但不明显;与预计相反,各预处理使SOD活性显著降低。结论:七氟烷预处理减轻脑氧化应激而具有脑保护作用,并与时间、剂量相关。
Aim:To investigate the effect of Sevoflurane (Sevo) preconditioning on oxidative stress of brain in acute hypoxia mice, and the relationship with duration of adminstering drug and concentration. Methods: Sixty male C56BL/6J mice were randomly allocated to 6 groups : Control, Hypoxia ( H, 6. 5% 02for 24 h) ,2% Sevo 30 rain + H, 2% Sevo 60 min + H, 4% Sevo 30 min + H,Propofol + H. According to the kit instruction steps, the brain was homogenized for the measurement of malondialdehyde ( MDA), superoxide dismutase ( SOD), glutathione peroxidase (GPx). Results: Compared with H group, the brain levels of MDA reduced in all Sevo precondition groups, moreover,in the 2% Sevo 60 min + H group and 4% Sevo 30 min + H group, significantly reduced, however Propofol + H group was not statistically signif- icant; the activity of GPx increased in three Sevo precondition groups, and in the 4% Sevo 30 min + H group, this increased significantly, activity of GPx reduced in the Propofol + H group, but has not significant ; the activity of SOD decreased in all precondition groups which was not influenced as expected. Conclusion:Sevo preconditioning protects the mouse brain against oxidative stress, and the protection is related to duration of adminstering drug and dose.
出处
《暨南大学学报(自然科学与医学版)》
CAS
CSCD
北大核心
2013年第4期405-409,436,共6页
Journal of Jinan University(Natural Science & Medicine Edition)
基金
广东省科技计划资助项目(2009B030801032)
关键词
七氟烷
预处理
缺氧
脑保护
氧化应激
sevoflurane
preconditioning
hypoxia
brain protection
oxidative stress