Notch1、MMP-2在三阴乳腺癌中的表达及意义

Expression and Significance of Notch1 and MMP-2 in Human Triple-negative Breast Cancer

【目的】探讨三阴乳腺癌组织（TNBC）中Notchl、基质金属蛋白酶-2（MMP-2）表达及其意义。【方法】采用免疫组织化学S-P法检测56例TNBC组织和30例癌旁正常乳腺组织Notchl、MMP-2的表达情况并比较。【结果】TNBC组织中Notchl、MMP-2阳性表达定位于细胞质和（或）细胞核，其表达率分别为62．50％（35／56）、53．57％（30／56），明显高于癌旁正常组织（P〈0．01）；56例TNBC组织中Notchl、MMP-2蛋白在组织学分级G3级阳性表达率高于G1＋G2级（P〈0．05），Notchl、MMP-2的病理分期Ⅲ～Ⅳ期的阳性表达率高于I～Ⅱ期（P〈0．05），Notchl、MMp-2阳性表达与淋巴结转移有关（P〈0．05）；相关性分析显示二者之间呈正相关（P〈O．05）。【结论】Notchl、MMP-2在TNBC中呈明显高表达，与TNBC的发生、发展和转移密切相关。

[ObjectivelTo explore the expression of Notchl and MMP-2 in human triple-negative breast cancer（TNBC） and its significance. [MethodslThe expressions o{ Notchl and MMP-2 in 56 TNBC tissues and 30 normal breast tissues adjacent to the cancer were examined and compared by using SP immunohistochemis- try method. [ResultsI The expressions of Notchl and MMP-2 in TNBC tissues were located in cytoplasm and/ or nucleus, and the positive expression rates were 562.50% （35/56） and 53.57% （30/56） respectively which were obviously higher than those in normal tissues adjacent to the cancer（ P 〈0.05）. In 56 TNBC tissues, the positive expressions of Notchl and MMP-2 in histological grade G~ were higher than those in G1＋G2 （ P 〈 0.05）. In addition, the positive expressions of Notch1 and MMP-2 in stage II- IV tumors were also higher than those in stage I-II tumors（ P 〈0.05）. Furthermore, the expressions of Notchl and MMP-2 were as- sociated with axillary lymph node metastasis（ P 〈0.05）. Correlative analysis showed a positive relation be- tween Notchl and MMP-2（ P 〈0.05）. [Conclusion] Notch1 and MMP-2 are highly expressed in TNBC, and their expressions are closely related with the occurrence, development and metastasis of TNBC.