球囊损伤腹主动脉大鼠血浆NO水平的变化及WSC对其影响

Changes in the balloon injury in rat plasma NO level of the abdominal aorta and the WSC its impact

目的探讨NO在RS中的作用及WSC对其影响。方法将54只大鼠分为模型组、假手术组和WSC组,每组18只。其中,模型组和WSC组大鼠应用球囊导管拉伤法建立大鼠主动脉内皮损伤模型,假手术组大鼠仅分离结扎右髂总动脉。WSC组大鼠均于造模后开始灌胃给药,剂量为200mg/kg,1ml/次(50mg/ml),每日1次,模型组、假手术组在同等情况下给予蒸馏水,连续3周。各组按7d、14d、21d3个时间点处死动物并静脉采血2ml,离心取上清-20℃保存待测。N0测定用硝酸还原酶法。结果第7d、第14d、第21d模型组的NO水平均明显低于假手术组,两者相比有极显著性差异(P<0.01),其各组之间差异不显著(P>0.05)。第14d和第21dWSC组的NO水平明显升高,与模型组相比有显著性差异(P<0.05,P<0.01),其中第21d与第7d相比差异极显著(P<0.01),而与假手术组相比无显著性差异(P>0.05)。结论 NO参与了球囊损伤大鼠腹主动脉RS的病理过程,WSC对球囊损伤大鼠腹主动脉RS有保护作用。

Objective Explore the role of NO in the RS and the WSC its impact.Methods 54rats were randomly divided into model group,sham operation group,and WSC group（n=18）.Among them,the model group and WSC rats application balloon catheter injury law to establish a rat aortic endothelial injury model,sham-operated rats only separated in the ligation of the right common iliac artery.WSC rats after modeling gavage at a dose of 200mg / kg,1ml /（50 mg / ml）,day 1,the model group,sham operation group under the same circumstances treated with distilled water,3 weeks in a row.Each group 7d,14d,21dthree time points animals were sacrificed and 2ml of venous blood collection,centrifugation the supernatant was stored at-20℃test.The N0determination of nitrate reductase method.Results The 7th day,14th day,the first 21dmodel group,NO was significantly lower than the sham group,both compared to a very significant difference（P0.01）,the difference between the groups was not significant（P0.05）.14dand the WSC 21dgroup,NO levels were significantly elevated compared with model group,significant differences（P0.05,P 0.01） difference between 21dand 7dhighly significant（P0.01）.compared with the sham group,no significant difference（P0.05）.Conclusion NO is involved in the pathological process of the balloon injury of rat abdominal aorta RS,the WSC restenosis balloon injury of rat abdominal aorta have a protective effect.