载20(S)-人参皂苷白蛋白纳米微球对人宫颈癌Hela细胞生长抑制作用研究

Anti-proliferation effects of 20(S)-ginsenoside Rg3 loaded human albumin nanopaticles(SPG-Rg3-HAS-NP)on human cervical cancer Hela cells inv itro

目的观察载20(S)-人参皂苷白蛋白纳米微球(SPG-Rg3-HAS-NP)对人宫颈癌Hela细胞体外增殖抑制作用。方法以人宫颈癌Hela细胞为研究对象,分为4个给药组:生理盐水对照组、空白白蛋白纳米微球组、20(S)-人参皂苷组和载20(S)-人参皂苷白蛋白纳米微球组;采用MTT法观察给予不同浓度药物后24h、48h、72h和96h4个时间点人宫颈癌Hela细胞生长抑制率。结果检测结果显示,空白白蛋白纳米粒对Hela细胞无明显细胞毒作用,且不具有时间和浓度依赖性。SPG-Rg3和SPG-Rg3-HAS-NP对宫颈癌Hela细胞均具有显著的细胞生长抑制作用,且Hela细胞生长分别与两组药物的给药浓度和时间呈一定的浓度依赖性和时间依赖性。SPG-Rg3组在80μg/ml浓度以后似已进入平台期甚至出现曲线下行的趋势,而SPG-Rg3-HAS-NP组的细胞生长曲线仍继续上升,72h后于20μg/ml浓度处抑制率已超过SPG-Rg3组(P<0.05)。提示SPG-Rg3-HAS-NP组药物对Hela细胞的抑制有一定的时间延续性,表明药物具有一定的缓释作用。结论 SPG-Rg3-HAS-NP对宫颈癌Hela细胞具有显著的体外细胞生长抑制作用,呈一定的浓度依赖性和时间依赖性。

Objective To study the Inhibitory effects of 20（S）-ginsenoside Rg3loaded human albumin nanopaticles（SPG-Rg3-HAS-NP）on human cervical cancer Hela cells in vitro.Methods Hela cells were treated with normal saline、blank human albumin nanopaticles、pure 20（S）-ginsenoside Rg3（SPG-Rg3）and 20（S）-ginsenoside Rg3loaded human albumin nanopaticles（SPG-Rg3-HAS-NP）respectively.Hela cells growth inhibition rates were detected after 24h、 48h、72hand 96hbeing treated with different drug concentration by MTT assay.Results Blank human albumin nanopaticles had no cytotoxicity on Hela cells without concentration-dependence and time-dependence in vitro.SPG-Rg3 group SPG-Rg3-HAS-NP group had significant anti-proliferation effect on Hela cells growth by MTT assay,and Hela cells growth had respectively concentration-dependence and time-dependence to SPG-Rg3group SPG-Rg3-HAS-NP group.Hela cells growth seemed to be platform phase and even have a down curve tendency after concentration of 80 μg/ml SPG-Rg3in SPG-Rg3group,but Hela cells growth had a continue ascend curve in SPG-Rg3-HAS-NP group.The SPG-Rg3-HAS-NP group had higher inhibitory effect than SPG-Rg3group after concentration of 20μg/ml SPGRg3and after 72hadministration（P0.05）.The above results indicated anti-proliferation effect on Hela cells growth in SPG-Rg3-HAS-NP group had a certain time last and the SPG-Rg3-HAS-NP had a certain sustained-releasing effect.Conclusion SPG-Rg3-HAS-NP has a significant anti-proliferation effect on human cervical cancer Hela cells with concentration-dependence and time-dependence in vitro.