氧化乐果长期暴露对大鼠骨骼肌氧化损伤的研究

Influence on oxidative injury in skeletal muscle tissue induced by omethoate in rats

目的探讨氧化乐果对大鼠骨骼肌组织氧化损伤的影响。方法将40只雄性Wistar大鼠随机分为4组,正常对照组,低剂量组,中剂量组和高剂量组,每组10只。低剂量组,中剂量组和高剂量组每天分别给予1/20、1/10、1/5LD50的氧化乐果,采用灌胃的途径给药,连续给药8周,检测大鼠血糖水平,骨骼肌组织中丙二醛(Malondialde-hyde,MDA)含量、超氧化物歧化酶(Superoxide dismutase,SOD)、过氧化氢酶(Catalase,CAT)和谷胱甘肽过氧化物酶(Glutathione peroxidase,GSH-Px)的活性以及骨骼肌的组织病理学检测。结果与正常对照组相比,低剂量、中剂量和高剂量组大鼠骨骼肌MDA含量显著升高(P<0.05),SOD、CAT和GSH-PX活性显著降低(P<0.05);大鼠的血糖和组织病理学检查未见异常变化。结论长期接触有机磷农药氧化乐果可导致骨骼肌出现氧化应激。

Objective To Explore the effects of omethoate on oxidative injury of skeletal muscle tissue in rats and to clarify the mechanism.Methods 40Wistar rats were randomly divided into four groups as normal control group,low dose group,middle dose group and high dose group.After treatment 8weeks,Fasting blood glucose and the histopathology of skeletal muscle tissue were detected.The contents of MDA and the activity of SOD,CAT and GSH-Px and the concentration of TC,TG,LDL and HDL in skeletal muscle tissue were detected.Results Compared with normal control group,the concentration of MDA of skeletal muscle tissue in low dose group,middle dose group and high dose group were significantly increased（P0.05）and the activity of SOD,CAT and GSH-Px were significantly decreased（P0.05）.Conclusion These results confirm omethoate can induce oxidative injury of skeletal muscle tissue.