Bax和TGFβRⅡ基因在肺癌组织中的移码突变

Bax and TGFβRⅡ frameshift mutations in lung cancer

目的 探讨Bax和TGFβRⅡ基因在肺癌组织中的移码突变及其与微卫星改变的关系。 方法 用PCR 变性聚丙烯酰胺凝胶电泳 银染法 ,检测 5 0例肺癌组织Bax和TGFβRⅡ基因移码突变及微卫星改变。结果 Bax基因 (G) 8移码突变 12例 ,占 2 4% (12 / 5 0 ) ,主要是碱基丢失。TGFβRⅡ的 (A) 10 未发现移码突变。Bax移码突变与微卫星不稳定和 /或杂合性缺失有密切关系 (P <0 .0 5 )。结论 肺癌组织中存在Bax基因移码突变且与微卫星改变密切相关 。

Objective To explore frameshift mutations in the Bax and TGFβRⅡ genes and the relationship between frameshift mutations and microsatellite alterations in lung cancer. Methods Frameshift mutations and microsatellite alterations were detected in 50 primary lung cancer tissues by PCR, 8% denature polyacrylamide gel electrophoresis and silver staining. Results Bax gene (G) 8 frameshift mutations were detected in 12 cases (24%,12/50), and all were base deletion mutations. Bax frameshift mutations correlated with microsatellite alterations (P<0.05). TGFβRⅡ (A) 10 frameshift mutations were not found. Conclusion Bax gene frameshift mutations are found in lung cancer, and closely correlate with microsatellite alterations. Bax gene and microsatellite might play a certain role in carcinogenesis of lung cancer.