人类恶性肿瘤癌旁组织中端粒酶活性的表达意义

Expression and Significance of Telomerase Activity in the Tumor-Adjacent Noncancerous Tissues of Human Common Neoplasms

目的:探讨端粒酶在人类常见恶性肿瘤癌组织和癌旁组织中的活性表达及其表达的临床意义。方法:采用端粒重复序列扩增PCR酶联免疫吸附法检测了人胃癌11例,结肠癌14例,肝癌4例,乳腺癌9例及其相应的38例癌旁组织中的端粒酶活性。结果:在38例肿瘤组织中,有31例端粒酶呈阳性(81.6％);38例癌组织中,有8例阳性(21.1％)。癌组织和癌旁组织中端粒酶的表达均与肿瘤临床病理特征无关。癌旁组织存在端粒酶阳性表达的病例,病理上多伴有不典型增生或癌细胞的浸润转移。结论:端粒酶本质上是细胞增殖活性而非肿瘤的分子水平标记物;对癌旁组织中端粒酶阳性的患者加强观察有助于早期发现某些术后再发或复发的肿瘤。

Objective: To evaluate the role of telomerase activity in the carcinomas tissue and rumor - adjacent non-cancerous tissues of human neoplasms. Methods: Telomerase activity in tumor samples, including 11 gastric, 14 colonic, 4 hepatic and 9 breast cancers, was examined by a novel telomerase TRAP and PCR ELISA assay. Thirty - eight samples from tumor - adjacent tissues were also assayed. Results: Thirty - one of 38 (81.6%) tumor samples and 8 of 38(21,1%) adjacent tissue specimens were positive for telomerase activty regardless of tumor size, histologieal grade and tumor stage. Almost all telomerase postive tumor - adjacent noncancerous exist displasia, infiltration and/or metastasis. Conclusion: Telomerasea is not a special tumor marker. In essential, it may just be a good molecular biomarker for tumor proliferation. Detection of telomerase activity in resected noncancerous tissues may be helpful in predicting postoperative recurrence and /or malignant transformation.