乙肝病毒X基因与人肝细胞癌关系的研究

Study on Relation between Hepatitis B Virus X Gene and Primary Hepatocellular Carcinoma

目的 探讨乙肝病毒 (HBV) X基因与肝癌发病的关系 ,为从分子水平上研究肝癌病因提供有关信息。方法 采用 PCR技术对 HBV不同状态的 1 0 2例原发性肝细胞癌 (PHC)、36例肝硬化 (L C)及 2 8例慢性肝炎 (CH)血清中的 HBVX基因进行了检测。结果 在 HBV5项标志均阴性的 53例 PHC检出 X基因 2 0例 (37.7% ) ,31例 LC中检出 5例 (1 6.1 % ) ,1 1例 CH中检出 2例 (1 8.2 % )。所有HBV X基因阳性血清 HBV S基因扩增产物检测结果 ,31例 PHC中检出 S基因阴性 2 0例 (64.5% ) ,8例 LC中检出 5例 (62 .5% ) ,1 1例 CH中全部为阳性。而在肝血管瘤和肝囊肿等其他肝病中未发现 X基因 (0 /35)。结论 HBV X基因整合于肝细胞染色体后与肝硬化、肝癌的发生发展关系密切 ;整合的 HBV X基因与p53抑癌基因的失活密切相关 ,HBV X基因的整合可能是肝癌发生的一个重要原因。

Objective To approach the relation between HBV X gene and hepatocellular carcinoma in order to provide informations for etiological study of hepatic cancer in molecule levels. Methods The serums from 102 cases of primary hepatocellular carcinoma(PHC),36 cases of liver cirrhosis(LC) and 28 cases of chronic hepatitis(CH) were detected for the HBV X gene. Results 20 X gene positive were detected in 53 samples of PHC without HBV serological marker(37.7%),5 in 31 LC(16.6%) and 2 in 11 CH(18.2%). In another study on all the samples whose X gene were positive 20 S gene negative samples in 31 X gene positive PHC(64.5%), 5 in 8 LC(62.5%) and none in 11 CH were found. Conclusion HBV X gene integrated with hepatocellular chromosome was closely relative to development of LC and PHC; Integrated HBV X gene was closely related to deletion of p53 inhibiting cancer gene, and the integration of HBV X gene may be an important cause of hepatic cancer.