子宫颈上皮内瘤变及鳞癌组织Survivin与C-myc蛋白表达及其意义

EXPRESSIONS OF SURVIVIN AND C-MYC PROTEIN IN CERVICAL INTRAEPITHELIAL NEOPLASIA AND SQUAMOUS CELL CARCINOMA

目的探讨Survivin、C-myc蛋白在子宫颈上皮内瘤变(CIN)及鳞癌(SCC)组织中表达的意义。方法应用免疫组化PV-6000及PV-9003法,分别检测60例CIN、40例SCC及10例非瘤变组织中Survivin、C-myc蛋白的表达情况。结果 Survivin、C-myc蛋白在非瘤变组织中均无表达;CIN及SCC组Survivin及C-myc的阳性表达强度均显著高于非瘤变组(Hc=23.209、21.315,q=18.086～26.022,P均<0.01),随CIN分级的增高,Survivin、C-myc的阳性表达强度逐渐增高(Hc=45.633、36.383,q=6.331～11.498,P均<0.01);CINⅢ组与SCC组、CINⅠ组与非瘤变组比较,Survivin及C-myc的阳性表达强度差异亦有显著性(q=6.078～26.156;P均<0.01)。子宫颈SCC组织中Survivin及C-myc蛋白的阳性率在高中、低分化组间、淋巴结有无转移组间及临床Ⅰ～Ⅱ、Ⅲ～Ⅳ期组间比较差异均有显著性(χ2=6.000～10.909,P<0.05)。Survivin、C-myc蛋白在CIN及SCC组织中的表达均呈显著正相关(r=0.680、0.722,P均<0.01)。结论 Survivin、C-myc在子宫颈CIN和SCC的发生及进展过程中具有协同作用,两者联合检测对于判定CIN及SCC的生物学行为具有重要的意义。

Objective To explore the significance of survivin and C-myc protein expressions in cervical intraepithelial ne- oplasia （CIN） and squamous cell carcinoma （SCC）. Methods The expressions of survivin and C-myc protein in 60 cases of CIN and 40 of SCC were detected by immunohistochemistrical tecnique; and that in 10 cases of non-CIN by PV-6000 and PV-9003 me- thods. Results No expressions of survivin and C-myc were noted in non-CIN group. The positive expressions of survivin and C- myc in both CIN and SCC groups were much higher than that in the non-CIN group （He =23.209,21.315 ;q= 18.086--26.022 ;P〈 0.01）. The positive expressions of survivin and C-myc gradually 36.383 ;q = 6.331 - 11.498 ;P〈0.01）. The differences of positive increased along with the increase of CIN grading （He = 45.633, expressions of both survivin and C-myc between CINⅢ and SCC, as well as CIN Ⅰ and non-CIN were significant （q=6.078-26.156,P〈0.01）. The differences of positive rates of survivin and C- myc in SCC were significant in the following conditions： between well- and moderately-differentiated and poorly-differentiated group, between with or without lymph-node metastasis, and clinically, between stage 1-2 and stage 3-4 （χ2 =6.000--10.909,P〈 0.05）. Both survivin and C-myc were positively correlated with CIN and SCC （r=0.680,0.722;P〈0.01）. Conclusion Survivin and C-myc play a joint action in the occurrence and development of CIN and SCC, a combined detection of the two parameters is of great significance in assessment of biological behaviour of CIN and SCC.