胞二磷胆碱对大鼠脑缺血损伤后认知功能及海马神经元BCL-2,BAX表达和细胞凋亡的影响

Effects of Citicoline on the Rat's cognitive Function,Expression of Hippocampal Neuron BCL-2 and BAX and Apoptosis After Cerebral Dschemia Injury

探讨胞二磷胆碱对脑缺血损伤后大鼠认知功能和海马神经元BCL-2,BAX表达的影响.取健康Spra-gue-Dawley成年雄性大鼠60只,随机分为假手术对照组,缺血组和胞二磷胆碱组3组,每组20只.对缺血组和胞二磷胆碱组采用Zea Longa等法改良复制大脑中动脉狭窄(middle cerebral artery occlusion,MCAO)动物模型,通过Morris水迷宫系统观察各组大鼠学习记忆功能;免疫组化检测BCL-2,BAX表达,Tunel法检测神经元凋亡.胞二磷胆碱使Bcl-2蛋白表达从缺血对照组的39.88±5.41增加至55.13±8.17,BAX蛋白表达从缺血对照组的62.38±8.47下降至36.13±5.94,凋亡神经元从缺血对照组的18.67±3.86下降至14.67±4.25.胞二磷胆碱对脑缺血损伤后大鼠的神经保护作用可能是通过上调海马神经元Bcl-2蛋白、下调Bax蛋白的表达,减少海马神经元凋亡进而改善脑缺血损伤后大鼠的学习记忆能力.

This paper investigates the effects of citicoline on the rat＇s cognitive function and expression of hippocampal neuron BCL-2and BAX after cerebral ischemia injury.With the equal and random principle,sixty healthy male adult SpragueDawley rats were divided into three groups which include sham operation control group,and ischemia group and citicoline group,each group of 20.In the last two groups,middle cerebral artery occlusion model was established by the improved method of Zea Longa.Then we evaluate each group＇s learning and memory function through Morris water maze test,Immunohistochemistry was used to detect BCL-2and BAX expression,and Tunel method to detect neuron apoptosis.Compared with the ischemia group,the protein expression of BCL-2increased from（39.88±5.41） to（55.13±8.17）,the protein expression of BAX decreased from（62.38±8.47） to（36.13±5.94）,and the apoptosis of neurons dropped from（18.67±3.86） to（14.67 ±4.25） in the citicoline group.Citicoline can improve the learning and memory function of rats after cerebral ischemia injury.The mechanism to protect these rats＇nerves may improve expression of hippocampal neuron BCL-2protein,decrease Bax protein and reduce apoptosis of hippocampal neurons.