摘要
呋塞米是一种高效利尿剂,本实验主要探究其对碳酸酐酶的抑制效应.相比较乙酰唑胺而言,呋塞米在其浓度接近碳酸酐酶浓度时能使该酶基本失活.研究发现,呋塞米对碳酸酐酶的抑制效应表现为非竞争性抑制,其IC50为0.759μM,KI为0.61μM,乙酰唑胺的IC50为0.199μM,KI为0.099μM,表现为竞争性抑制.
The inhibitory effect of a high efficient diuretic,furosemide,on carbonic anhydrase was investigated in this study. Compared with acetazolamide, furosemide can quickly make BCA II inactive when its concentration is close to the enzyme concentration. The results show that furosemide is a non-competitive inhibitor of carbonic anhydrase, the vaules of its IC50 and K1 are 0. 759 μM, 0.51 μM. Acetazolamide is a competitive inhibitor of carbonic anhydrase, the vaules of its ICso and K1 are 0. 199 μM,0. 099 μM.
出处
《四川大学学报（自然科学版）》
CAS
CSCD
北大核心
2013年第4期887-891,共5页
Journal of Sichuan University(Natural Science Edition)
基金
863项目(2012AA02204)
关键词
碳酸酐酶
非竞争性抑制
酶动力学
呋塞米
carbonic anhydrase
noncompetitive inhibition
enzyme kinetics
furosemide
