痹痛康丸对胶原性关节炎大鼠TNF-α的影响

Effect of Bitongkang Pills on tumor necrosis factor - alpha in collagen - induced arthritis rats

目的观察痹痛康丸对患胶原性关节炎(collagen-induced arthritis,CIA)大鼠血清肿瘤坏死因子α(TNF-α)的影响,探讨其治疗作用机制。方法建立Ⅱ型胶原加完全弗氏佐剂诱导的大鼠CIA模型,60只Wistar大鼠随机分为6组,痹痛康丸低、中、高剂量组[0.3 g/(kg.d)、3 g/(kg.d)、30 g/(kg.d)],尪痹冲剂组3 g/(kg.d),甲氨蝶呤片组每周1.2 mg/kg,采用药物溶解于2 ml生理盐水中,1次/d,连续灌胃给药4周,模型组灌服生理盐水2 ml/只。8周后处死大鼠,采用酶联免疫吸附法(enzyme-linked immunosorbent assay,ELISA)检测CIA大鼠血清TNF-α浓度,观察各组大鼠TNF-α浓度水平的变化。结果痹痛康丸中剂量组TNF-α浓度水平(1190.84±177.02)、高剂量组TNF-α浓度水平(844.20±142.79)、MTX片组(1149.40±182.17)、尪痹冲剂组(1215.83±102.07)显著低于模型组(1724.67±247.26)(P=0.017,P=0.000,P=0.008,P=0.036)。痹痛康丸高剂量组血清TNF-α浓度水平显著低于痹痛康丸中剂量组、MTX片组、尪痹冲剂组,(P=0.006,P=0.018,P=0.009)。结论痹痛康丸能有效降低CIA大鼠血清TNF-α的表达,有抑制CIA大鼠滑膜炎性的作用。

Objective To study the effect of Bitongkang Pills on TNF-α of collagen-induced arthritis(CIA) rats’blood serum,explore its mechanism of therapeutic action.Methods The model of type II collagen with complete Freund’s adjuvant-induced rat CIA was established.60 Wistar rat models were randomly divided into six groups,Bitongkang Pill low,medium and high dose groups [0.3,3,30 g /(kg.d) ],costumes,Wang bi instant herbal medicine group 3 g /(kg.d),methotrexate tablets group 1.2 mg /(kg.w),the drugs of above groups were dissolved in 2 ml of saline,the rats were fed with the pills one time per day,intragastrically administered for 4 weeks continuously.The model group was fed with 2 ml saline each rat.After 8 weeks,the rats were sacrificed,underwent enzyme-linked immunosorbent assay(ELISA) to detect the CIA rat serum TNF-alpha concentration,and to observe each rat’s TNF-alpha concentration levels changes.Results The serum TNF-alpha levels in Bitongkang pills medium dose group(1190.84 ± 177.02),high-dose group(844.20 ± 142.79),MTX tablets group(1149.40 ± 182.17),Wangbi granules group(1215.83 ± 102.07) were significantly lower than the model group(1724.67 ± 247.26)(P = 0.017,0.000,0.008,0.036).The serum TNF-alpha levels in Bitongkang Pills high dose group were significantly lower than those in the Bitongkang Pills medium dose group,the MTX tablets group,and Wangbi granules group(P = 0.006,0.018,0.009).Conclusions Bitongkang Pills can effectively reduce the CIA serum TNF-alpha expression,have the action of inhibiting CIA rat synovitis.