乙酰唑胺对类风湿关节炎滑膜细胞增殖及TNF-α及IL-1β的影响

Effects of acetazolamide on proliferation,IL-1β expression,and TNF-α expression of fibroblast-like synoviocytes of rheumatoid arthritis

目的观察乙酰唑胺(AZ)对类风湿关节炎(RA)滑膜细胞(FLS)的增殖影响,及AZ对RA FLS培养上清中TNF-α和IL-1β水平的影响。方法从RA患者关节液中分离滑膜细胞体外培养,不同浓度AZ(10-4,10-6,10-8mol/L)处理RA FLS 24,48,72 h后,采用四甲基偶氮唑蓝(MTT)法检测AZ对RA FLS增殖影响;ELISA法检测AZ处理后培养上清中TNF-α和IL-1β水平。结果不同浓度AZ处理的RA FLS吸光度值比较无统计学差异(P>0.05),随着时间延长,各组间细胞增殖有抑制趋势,与不加药对照组相比差异有统计学意义(P<0.05);AZ可呈剂量及时间依赖性抑制RA FLS分泌TNF-α和IL-1β(P<0.05)。结论 AZ可以抑制RAFLS增殖及减少RA FLS分泌IL-1β和TNF-α。

To observe the effect of acetazolamide on proliferation,IL-1β expression,and TNF-α expression of fibroblast-like synoviocyte（FLS） of rheumatoid arthritis（RA）,FLS were separated from synovia and cultured in vitro.Then,RA FLS was treated with different concentrations of acetazolamide（10-4,10-6,10-8 mol/L） for different times（24,48,72 h）.We employed MTT method to study the growth inhibition of RA FLS,ELISA to detect the concentration of TNF-α and IL-1β in cell-cultured supernatant fluid.Our results showed there was no significant difference in absorbance value of RA FLS treated with different concentrations of acetazolamide（P 0.05）,but all concentrations of acetazolamide could inhibit the proliferation of RA FLS in a time-dependent manner（P 0.05）.Furthermore,different concentrations of acetazolamide could significantly inhibit the expression levels of IL-1β and TNF-α in cell-cultured supernatant fluid of RA FLS（P 0.05） in a time-dependent and dose-dependent manner.In conclusion,acetazolamide could inhibit the proliferation of RA FLS and decreased the secretion of TNF-α and IL-1β in cell-cultured supernatant fluid of RA FLS.