经门静脉药物灌注化疗栓塞治疗大鼠肝泡状棘球蚴病的电镜观察

Trans-portal vein chemoembolization for the treatment of alveolar echinococcosis in experimental rats: pathologic observation with electron microscope

目的观察并探讨经门静脉灌注阿苯达唑脂质体与碘化油混悬液后大鼠肝泡状棘球蚴组织的电镜下形态学变化过程。方法以1只正常饲养大鼠为空白对照组,1只感染泡球蚴病大鼠为实验对照组,19只复制成功模型大鼠为实验组分为三组。将19只模型大鼠开腹直视下穿刺门静脉,灌注阿苯达唑脂质体(0.1 ml)和碘化油(0.1 ml)混悬液共0.2 ml,分别在4、7、10 d后处死动物,采集标本,行电镜观察。结果灌注化疗栓塞后,4 d内泡球蚴周边组织结构以炎性改变为主,泡球蚴组织基本正常。7 d时可见泡球蚴组织结构发生变性改变,10 d可见肝泡球蚴组织结构破坏,囊泡塌陷,生发层、角质层结构退变等坏死表现。结论门静脉途径介入治疗肝泡状棘球蚴病是一种有效的治疗技术方法,疗效迅速、确切。

Objective To observe the pathologic changes of alveolar echinococcosis after trans-portal vein infusion of liposome entrapped albendazole and iodinated oil suspension with the help of electron microscope,and to discuss the therapeutic effect of this technique.Methods One normal rat was used as blank control group.One rat with alveolar echinococcosis was used as experimental control group.Laparotomy was performed in nineteen model rats,then,under direct vision the portal vein was punctured,which was followed by an injection of 0.2 ml suspension（0.1 ml liposome entrapped albendazole and 0.1 ml iodinated oil）.The experimental rats were divided into three groups.The rats of the three groups were sacrificed at 4,7 and 10 days after the procedure respectively.The specimens were collected and sent for examination with electron microscope.The pathological results were analyzed.Results Four days after the treatment,the main finding on microscopic examination was inflammatory reaction around the alveolar echinococcosis lesions,while the alveolar echinococcosis structure was basically normal.Seven days after the treatment,degeneration of alveolar echinococcosis tissue began to emerge.And ten days after the treatment,obvious structure destruction of alveolar echinococcosis,collapse of vesicae,degeneration or necrosis of germinal layer and horny layer,etc.could be seen.Conclusion For the treatment of alveolar echinococcosis in experimental rats,trans-portal vein interventional therapy is effective and reliable with prompt response.