TFF3和SDF-1在甲状腺乳头状癌中的表达及临床意义

Expression and Clinical Significance of TFF3 and SDF-1 Proteins in Papillary Thyroid Carcinoma

目的分析三叶因子3（TFF3）、基质细胞诱导因子-I（SDF-1）在甲状腺乳头状癌（PTC）和癌旁组织中的表达及意义，为临床诊断vrc及评估患者预后提供依据。方法采用免疫组织化学染色，检测并比较PTC组织芯片中癌与癌旁组织TFF3、SDF-1蛋白的表达，并分析二者的表达与临床病理特征的关系。结果（1）PTC中TFF3阳性率和表达水平明显高于癌旁组织，在有淋巴结转移患者明显高于无淋巴结转移患者，临床Ⅲ～Ⅳ期PTC患者高于I～Ⅱ期患者（P〈0．05）。（2）PTC中SDF-1的阳性率及表达水平明显高于癌旁组织，有淋巴结转移患者明显高于无淋巴结转移患者，临床Ⅲ一Ⅳ期患者高于I～Ⅱ患者；45岁以上患者明显高于≤45岁者（P〈0．05）。（3）PTC中TFF3与SDF-1蛋白表达水平呈正相关m=0．266，P〈0．05）。结论TFF3和SDF-1在vrc中的高表达与癌的发生发展有关，对判断PTC的恶性程度和病情进展有重要价值。

Objective To investigate the expression and significance of trefoil factor 3 （TFF3） and stromal cell de- rived factor-1 （SDF-1） in papillary thyroid carcinoma （PTC） and adjacent tissues, and to provide the basis for clinical diagno- sis and prognosis evaluation. Methods The expressions of TFF3 and SDF- 1 proteins in PTC and adjacent tissues were de- tected by immunohistochemical SP method. The relationship between expressions of TFF3, SDF- 1 and clinicopath01ogical features was analyzed. Results （1） The positive rate and the expression level of TFF3 protein were significantly higher in PTC than those in adjacent tissues, which were higher in patients with lymph node metastasis than those in patients without lymph nodes metastasis, and were higher in PTC patients of stage ]U/IV than those in patients of stage I / II （P 〈 0.05）. The strong positive rate of TFF3 protein in stage III/1Vof PTC patients was higher than that in stage I / II （P 〈 0.01 ）. （2） The posi- tive rate and the expression level of SDF- 1 protein were significantly higher in PTC than those in adjacent tissues, which were higher in patients with lymph node metastasis than those in patients without lymph nodes metastasis, and were higher in PTC patients of stage m/IV than those in patients of stage I /II, and were significantly higher in patients over 45 years old than those of patients ≤45 years old （P 〈 0.05）. （3） There was a positive correlation between expression levels of TFF3 and SDF- 1 proteins in PTC （r=0.266, P 〈 0.05）. Conclusion The higher expressions of TFF3 and SDF- 1 in PTC were closely correlated with carcinogenesis and progression, which may play a significant value in judging the malignant degree and progression of PTC.