多效生长因子对大鼠肝细胞生长及白蛋白分泌功能的影响

Pleiotrophin stimulates growth and albumin secretion of rat hepatocytes in culture

目的了解多效生长因子（PTN）对大鼠肝细胞生长及白蛋白（Alb）分泌功能的影响。方法根据培养液的不同，将体外培养的原代大鼠肝细胞分成3组，A组为空白对照组，B组加入3T3细胞培养上清液作为阳性对照组，C组加入人类重组PTN（hrPTN）作为实验组。比较3组肝细胞的生长情况，包括观察肝细胞的生长速度及分泌Alb的功能。组间差异采用单因素方差分析，各组之间的两两差异通过LSD法进行比较。结果A、B、C组肝细胞生长率分别为2．800％±0．084％、4．300％土0．1320／0、3．800％土0．053％，B组肝细胞生长率明显高于A组和C组（／7=333．735，P〈0．05）。3组肝细胞上清液的Alb峰值集中在第2～6天，第2、第4及第6天，A组细胞上清液的Alb值分别为（0．550±0．010）g／L、（0．900±0．030）g／L和（0．300士0．040）g／L，B组分别为（0．900±0．030）g／L、（1．300±0．020）g／L和（1．400±0．030）g／L，C组分别为（0．900±0．010）g／L、（1．160±0．010）g／L和（0．700±0．050）g／L，方差分析结果均表明，B组促进肝细胞合成Alb的能力最强（F值分别为651．355、338．831和863．205，尸值均〈0．05）。结论PTN能刺激肝细胞生长速度增陕及分泌Alb功能增强，对大鼠肝细胞体外培养有促进作用。

Objective To investigate the influence of pleiotrophin （PTN） on the growth of rat hepatocytes. Methods Primary rat hepatocytes were isolated from male Sprague-Dawley rats and divided into three groups： group A （negative control）, cultivated in normal culture medium; group B （positive control）, cultivated with culture medium supplemented with supernatant from the embryonic fibroblast 3T3 cell line; group C （experimetal）, cultivated with culture medium supplemented with human recombinant （hr）PTN （100 ng/ml）. The hepatocytes＇ growth rate and level of secreted albumin （ALB） were evaluated by microscopy and biochemical assay, respectively. Significance of between-group differences were assessed by one-way ANOVA, and pairwise comparisons were performed by the least significant difference test. Results The growth rates of hepatocytes in groups A, B and C were 2.800 ± 0.084%, 4.300 ± 0.132% and 3.800 ± 0.053%, respectively. The growth rate of group B was significantly higher than the other two groups （F = 333.735, P 〈 0.05）. For all groups, the highest levels of secreted ALB were detected between the second and sixth day of culture, with g/L concentrations at day 2, 4 and 6 of： group A, 0.550 ± 0.010, 0.900 ± 0.030 and 0.300 ± 0.040; group B, 0.900 ±0.030, 1.300 ± 0.020 and 1.400 ± 0.030; group C, 0.900 ± 0.010, 1.160 ± 0.010 and 0.700 ± 0.050. The secreted ALB of group B was significantly higher than that of the othertwo groups （F = 651.355, 338.831 and 863.205, P 〈 0.05 ）. Conclusion PTN can benefit in vitro culturing of rat hepatocytes by stimulating growth and enhancing their ability to secrete albumin.