BMP2的表达对联合培养体系中骨髓间充质干细胞增殖和成骨分化的影响

BMP2 expression impacts the growth and osteogenic differentiation of BMSCs in the co-culture system

目的研究BMP2在hBMSCs和hUVECs体外联合培养系统中对细胞增殖和成骨分化的调控作用。方法选用第三代hUVECs、hBMSCs细胞以及经BMP2特异性静默干扰处理的hUVECs细胞建立联合培养体系。观察不同培养组单独hBMSCs组、hUVECs与hBMSCs细胞联合培养组以及经BMP2蛋白静默干扰处理hUVECs与hBMSCs细胞联合培养干扰组中BMSCs数量，运用酶活性法和放射免疫法分别对碱性磷酸酶及骨钙素的表达进行检测。结果在BMP2静默的hUVECs联合hBMSCs培养干扰组观察到随BMP2蛋白分泌减少，hBMSCs的细胞数目下降，但仍高于单独hBMSCs培养组，差异有统计学意义（P〈0．05）。联合培养体系中运用RNA干扰技术静默血管内皮细胞对BMP2的表达后，联合培养干扰组内hBMSCs对碱性磷酸酶、骨钙素在同一时间点表达低于联合培养组，但仍然高于单独问充质干细胞培养组，差异有统计学意义（P〈0．05）。结论联合培养体系中血管内皮细胞分泌的BMP2在BMSCs增殖和成骨分化诱导方面起着重要作用。

Objective To investigate the effects of BMP2 in the co-culture system of hBMSCs and hUVECs. Methods Based on the co-culture system of cells, a simple culture group （hBMSCs group） , a combined culture group （BMSCs ＋ hUVECs group ） and a BMP2 silenced group （BMSCs ＋ BMP2 silenced hUVECs） were established. On days 4,6,8,10, cells were counted for drawing cell growth curves. In addition, ALP and OC expression in BMSCs was detected in each group. Results At each time point, cell number in the BMSCs ＋ hUVECs group were significantly greater than in the BMSCs group, while the BMP2 silenced group were in the median. ALP and OC expression in the BMSCs ＋ hUVECs group were significantly greater than in the BMSCs group, while the BMP2 silenced group were in the median. Conclusion BMP2 plays an important role in the co-culture system, which can promote osteogenic differentiation and the growth of BMSCs.