颅底脊索瘤Brachyury、Galectin-3与临床特征相关性研究

The relationshipbetween Brachyury, Galectin - 3 expression in skull base chordoma and clinical characteristics

目的探讨颅底脊索瘤Brachyury和Galectin-3蛋白表达与患者临床特征的相关性。方法回顾性分析28例经手术治疗的颅底脊索瘤患者的临床资料并进行随访，采用免疫组化方法对收集的标本行Brachyury和GMectin-3蛋白检测。结果28例中经典型23例，软骨型1例，未分化型4例，Brachyury和Galectin-3蛋白免疫组化染色均为阳性。未分化型Brachyury的表达低于经典型（P=0．011），未分化型Galectin-3蛋白表达高于经典型（P=0．018）。结论Brachyury和Galectin-3蛋白表达水平与病理类型相关，恶性度高的未分化型Brachyury表达低于其他两型，GMectin-3蛋白在未分化型中表达高于经典型。Brachyury和Galectin-3蛋白表达水平可作为预测患者预后的参考因素。

Objective The goal of this study was to report on the expression of Brachyury and Galectin- 3 protein in chordoma and their correlation with the patients＇ clinical characteristics. Methods The author retrospectively studied data from 28 patients with skull - base chordoma who had undergone surgeries in the Department of Skullbase and Brain Stem of Beijing Tiantan Hospital between 2005 and 2012, and follow up was performed through recheck in out- patient department, telephone and letter. Paraffin - embedded chordoma specimens of these patients was made after their surgeries, the pathological type can be confirmed by hematoxylin - eosin （HE） staining, Brachyury and Galectin - 3 expression was investigated by immunohistochemistry. Spss 13.0 was used to analysis the related data. Results In these 28 specimens, 23 are typical type, 1 is chondroid type and 4 are atypical type, all specimens contain chordoma tumor cells whichexpressbrachyury and Galeetin - 3 strongly. Brachyury protein level is lower in atypical chordomas than that in typical chordomas （ P = 0. 011 ） and chondroid chordoma （ P = 0. 004 ）, while the atypical ones express higher level Galectin -3 than typical chordomas （P = 0. 018 ）. Conclusions All specimens expressed Brachyury and Galectin - 3 strongly, and the expression level of these two proteins have no correlation with patients＇ age, sex, history of treatment and size of tumor while correlate obviously with their pathologies. The atypical ehordoma is comparatively more malignant than typical ehordoma and ehondroid ehordoma. Brachyury level is lower in atypical chordomas than that in the other two types ; Gal - 3 protein level is higher in atypical chordomas than that in the typical ones. Brachyury and Gal- 3 protein level of the tumor are important factors in forecasting patients＇ prognosis.