期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

HPV16 E2对宫颈癌SiHa细胞上皮间质转化的影响 被引量：1

Influence of HPV16 E2 on epithelial-mesenchymal transition of cervical cancer SiHa cells
下载PDF
导出
摘要 目的探讨HPV16 E2对宫颈癌SiHa细胞上皮间充质细胞转化的影响。方法采用脂质体转染p-CAG-MYC-HPV16 E2至宫颈癌SiHa细胞中,倒置相差显微镜下观察转染前后细胞形态的改变;RT-PCR及Western blotting检测转染前后Twist2、上皮细胞标志物E-cadherin及间质细胞标志物Vimentin表达的变化。结果成功转染HPV16 E2至宫颈癌SiHa细胞中。转染后,上皮细胞由椭圆形变成梭形,细胞间隙增大;Twist2和Vimentin表达上调,E-cadherin表达下调。结论 HPV16 E2能在体外诱导宫颈癌细胞发生上皮间充质细胞转化,促进早期癌变。 Objective To investigate the influence of HPV16 E2 on epithelial-mesenchymal transition of cervical cancer SiHa ceils. Methods Lipofectamine was used to transfect p-CAG-MYC-HPV16 E2 gene into cervical cancer SiHa cells. The morphology of cells was observed before and after transfection with phase-contrast microscope, and the expression of Twist2, E-cadherin and Vimentin before and after transfection was determined by RT-PCR and Western blotting. Results HPV16 E2 was successfully transfected into SiHa cells. After transfection, the epithelial cells became spindle-shaped, with enlarged intercellular space, and the expression of Twist2 and Vimentin was up-regulated, while the expression of E-cadherin was down-regulated. Conclusion HPV16 E2 can induce epithelial-mesenchymal transition of cervical cancer cells, and promote cervical carcinogenesis.
作者 钱文艳 张稼闻 董煜 刘源 石灿 张箴波 杨永彬 丰有吉 邬素芳
机构地区 上海交通大学附属第一人民医院妇产科 昆山中医院
出处 《上海交通大学学报（医学版）》 CAS CSCD 北大核心 2013年第8期1074-1078,共5页 Journal of Shanghai Jiao tong University:Medical Science
基金 上海市科委项目(09411962500) 上海市科委浦江人才计划D类项目(12PJD002) 国家自然基金青年项目(81201541) 卫生部行业基金项目(201002013)~~
关键词 HPV16 E2 上皮间质转化 转染 宫颈癌 HPV16 E2 epithelial-mesenchymal transition transfection cervical cancer
分类号 R737.33 [医药卫生—肿瘤]
  • 相关文献

参考文献18

  • 1Faridi R, Zahra A, Khan K, et al. Oncogenic potential of Human Papillomavirus (HPV) and its relation with cervical cancer [ J]. Virol J, 2011, 8: 269.
  • 2Lee MY, Chou CY, Tang M J, et al. Epithelial-mesenchymal transi- tion in cervical cancer: correlation with tumor progression, epidermal growth factor receptor overexpression, and snail up-regulation [J]. Clin Cancer Res, 2008, 14(15): 4743 -4750.
  • 3Lee MY, Shen MR. Epithelial-mesenchymal transition in cervical carcinoma[J]. Am JTransl Res, 2012, 4(1): 1 -13.
  • 4Iwatsuki M, Mimori K, Yokobori T, et al. Epithelial-nxesenehymal transition in cancer development and its clinical significance [ J ]. Cancer Sci, 2010, 101(2) : 293 -299.
  • 5Sanchez-Tillo E, Liu Y, de Barrios O, et al. EMT-activating tran- scription factors in cancer: beyond EMT and tumor invasiveness [J]. CellMol Life Sci, 2012, 69(20): 3429 -3456.
  • 6Chamulitrat W, Sattayakhom A, Herold-Mende C, et al. Human papillomavirus 16 E6/ET-immortalized human gingival keratinocytes with epithelial mesenchymal transition acquire increased expression of cIAP-1, Bclx and p27 ( Kipl ) [ J]. Exp Dermatol, 2009, 18 (12) : 1067 -1069.
  • 7Caberg JH, Hubert PM, Begon DY, et al. Silencing of E7 oncogene restores functional E-cadherin expression in human papillomavirus 16-transformed keratinocytes [J]. Carcinogenesis, 2008, 29 ( 7 ) : 1441 - 1447.
  • 8Chamulitrat W, Schmidt R, Chunglok W, et al. Epithelium and fibroblast-like phenotypes derived from HPV16 E6/ET-immortalized human gingival keratinocytes following chronic ethanol treatment [J]. Eur J Cell Siol, 2003, 82(6): 313-322.
  • 9Cheng YM, Chou CY, Hsu YC, et al. The role of human papillo- mavirus type 16 E6/E7 oncoproteins in cervical epithelial-mesenchy- mal transition and carcinogenesis [J]. Oncol Lett, 2012, 3 ( 3 ) : 667 - 671.
  • 10Thierry F. Transcriptional regulation of the papillomavirus oncogenes by cellular and viral transcription factors in cervical carcinoma[ J ]. Virology, 2009, 384(2): 375-379.

二级参考文献20

  • 1Schluth-Bolard C,Till M,Labalme A,et al.TWIST microdeletion identified by array CGH in a patient presenting Saethre-Chotzen phenotype and a complex rearrangement involving chromosomes 2 and 7[J].Eur J Med Genet,2008,51 (2):156-164.
  • 2Pan D,Fujimoto M,Lopes A,et al.Twist-1 is a PPARdeha-in-ducible,negative-feedback regulator of PGC-lalpha in brown fat metabolism[J].Cell,2009,137(1):73-86.
  • 3Lopez D,Niu G,Hnber P,et al.Tumor-induced upregulation of Twist,Snail,and Slug represses the activity of the human VE-cad-herin promoter[J].Arch Biochem Biophys,2009,482(1/2):77-82.
  • 4Ansieau S,Bastid J,Doreau A,et al.Induction of EMT by twist pro-teins as a collateral effect of tumor-promoting inactivation of prema-ture senescence[J].Cancer Cell,2008,14(1):79-89.
  • 5Shiota M,Izumi H,Onitsuka T,et al.Twist and p53 reciprocally regulate target genes via direct interaction[J].Oncngene,2008,27(42):5543-5553.
  • 6Cheng GZ,Zhang WZ,Coppola D,et al.Twist is transcriptionally induced by activation of STAT3 and mediates STAT3 oncogenic function[J].J Biol Chem,2008,283(21):14665-14673.
  • 7Hong KO,Kim JH,Hong JS,et al.Inhibition of Akt activity induces the mesenchymal-to-epitbelial reverting transition with restoring E-cadherin expression in KB and KOSCC-25B oral squamous cell carcinoma cells[J].J Exp Clin Cancer Res,2009,28:28.
  • 8DiMeo TA,Anderson K,Phadke P,et al.A novel lung metastasis signature links Wnt signaling with cancer cell self-renewal and epithelial-mesenchymal transition in basal-like breast cancer[J].Cancer Res,2009,69(13):5364-5373.
  • 9Lee YB,Bantounas I,Lee DY,et al.Twist-1 regulates the miR-199a/214 cluster during development[J].Nucleic Acids Res,2009,37(1):123-128.
  • 10Gort EH,van Haaften G,Verlannl I,et al.The TWIST1 oncogene is a direct target of hypoxia-inducible factor-2α[J].Oncogene,2008,27(11):1501-1510.

共引文献2

同被引文献21

  • 1Pieeoni MA. Human papilomavirus detection in cervical cancer prevention [ J ]. Medicina ( B Aires), 2013, 73 ( 6 ) : 585 - 596.
  • 2Schrevel M, Gorter A, Kolkman-Uljee SM, et al. Molecular mecha- nisms of epidermal growth factor receptor overexpression in patients with cervical cancer[ J. Mod Pathol, 2011, 24(5) : 720 -728.
  • 3Ikari A, Sato T, Watanabe R, et al. Increase in claudin-2 expres- sion by an EGFR/MEK/ERK/c-Fos pathway in lung adenocarcinoma A549 cells[J]. Biochim Biophys Acta, 2012, 1823(6): 1110- 1118.
  • 4Kastritis E, Bamias A, Bozas G, et al. The impact of age in the out- come of patients with advanced or recurrent cervical cancer after platinum-based chemotherapy[ J]. Gynecol Oncol, 2007, 104(2) : 372 -376.
  • 5Gadducci A, Guerrieri ME, Greco C, et al. Tissue biomarkers as prognostic variables of cervical cancer[ J]. Crit Rev Oncol Hematol, 2013, 86(2) : 104 -129.
  • 6Lim K, Small W Jr, Portelance L, et al. Consensus guidelines for delineation of clinical target volume for intensity-modulated pelvic radiotherapy for the definitive treatment of cervix cancer [ J]. Int J Radiat Oncol Biol Phys, 2011, 79(2) : 348 -355.
  • 7Gumuskaya B, Alper M, Hueumenoglu S, et al. EGFR expression and gene copy number in triple-negative breast carcinoma [ J ]. Cancer Genet Cytogenet, 2010, 203 (2) : 222 -229.
  • 8Jutten B, Rouschop KM. EGFR signaling and autophagy depen- dence for growth, survival, and therapy resistance[ J]. Cell Cycle, 2014, 13(1): 42-51.
  • 9Iida K, Nakayama K, Rahman MT, et al. EGFR gene amplification is related to adverse clinical outcomes in cervical squamous cell carcinoma, making the EGFR pathway a novel therapeutic target [J]. BrJCancer, 2011, 105(3): 420 -427.
  • 10Ueno S, Sudo T, Oka N, Absence of human papillomavirus infection and activation of PI3K-AKT pathway in cervical clear cell carcinoma [J]. Int JGynecolCancer, 2013, 23(6): 1084-1091.

引证文献1

二级引证文献4

上海交通大学学报（医学版）
2013年 第8期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部