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Real-time PCR技术在弧菌DNA-DNA同源性测定中的应用

DETERMINATION OF DNA–DNA RELATEDNESS IN VIBRIOS BY MEANS OF REAL-TIME PCR
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摘要 目前在微生物学领域,研究各级分类单元的最有效手段是多相分类,该法能较客观地反映生物间自然系统进化的关系。DNA-DNA同源性分析在多相分类中扮演重要的角色,常用于亲缘关系密切的微生物种内相似度描述。它在检测错分菌株以及新描述的菌株划归已有分类单元时非常有用。DNA-DNA同源性分析最常用的检测手段是点杂交,杂交值可反映出两基因组间序列的相似度。已有研究证明,基因组碱基对每错配1%,
作者 金春英
机构地区 华侨大学化工学院
出处 《水生生物学报》 CAS CSCD 北大核心 2013年第4期782-786,共5页 Acta Hydrobiologica Sinica
基金 国家自然科学基金项目(No.31272684) 中国大洋协会项目(DY125-15-T-08) 国家海洋局海洋生物遗传资源重点实验室开放研究基金项目(编号:HY201102)资助
关键词 DNA-DNA同源性 点杂交 弧菌 SYBR Green I 热变性温度 DNA-DNA relatedness Dot blot hybridization Vibrio SYBR Green I Thermal denaturation temperature
分类号 Q939.112 [生物学—微生物学]
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参考文献17

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共引文献17

水生生物学报
2013年 第4期

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