慢性酒精中毒对大鼠视功能的影响及抗氧化剂ebselen的保护作用

The effects of chronic alcoholism on visual function in rats and the protective effect of ebselen

目的探讨慢性酒精中毒对大鼠视功能的损害以及抗氧化剂ebselen对视功能的保护作用。方法实验研究。利用自由饮方法建立慢性酒精中毒大鼠模型，按随机数字表将60只大鼠随机分为对照组、酒精组和ebselen治疗组（即药物组），采用闪光视网膜电图（FERG）及闪光视觉诱发电位（FVEP）观察大鼠视功能的改变。对数据进行单因素方差分析。结果3组FERGa、b波潜时差异有统计学意义（F=4．161、3．664，P〈0．05），酒精组a、b波潜时较对照组明显长（P〈0．05），而药物组与对照组相比差异无统计学意义（P〉0．05）。FERGb波振幅酒精组及药物组均较对照组低（F=3.312，P〈0．05）。3组FVEPN1波、P1波潜时差异有统计学意义（F=7．940、5．07，P〈0．05），与对照组相比，酒精组N1波、P1波潜时明显较长（P〈0．05）。3组FVEPN1-P1波振幅差异有统计学意义（F=3．874，P〈0．05）．与对照组相比，N1-P1波振幅明显较低（P〈0．05）。药物组N1、P1波潜时及N1-P1振幅与对照组相比差异均无统计学意义（P〉0．05）。结论慢性酒精中毒对大鼠视功能产生显著损害，抗氧化剂ebselen对视功能具有保护作用，证实氧化应激反应在慢性酒精中毒损害机制中发挥了重要作用。

Objective To evaluate the effects of chronic alcoholism on visual function in rats and the protective effects of ebselen. Methods In this experimental study, the models were based on chronic alcohol feeding. Rats were divided randomly into 3 groups： control group, ethanol group and ethanol＋ebselen group （drug group）. Visual function was assessed by FERG and FVEP. Data were analyzed using ANOVA. Results The lateneies of the a-wave and b-wave of FERG were obviously prolonged in the ethanol group compared to the control group （F=4.161, 3.664, P〈0.05）, while no differences were found between the drug group and control group （P〉0.05）. The amplitude of the b-wave decreased in the ethanol and drug groups compared to the control group （F=3.312, P〈0.05）, For FVEP, the lateneies of the N1 and P1 waves were prolonged and the amplitude of the NI-P1 wave obviously decreased in the ethanol group compared to the control group （F=7.940, 5.0707, 3.874, P〈0.05）, while no differences were found between the drug group and control group （P〉0.05）. Conclusion The damage to the visual system from chronic alcoholism can be prevented by an antioxidant. Thus, it can be inferred that oxidative stress plays a significant role in the damage from chronic alcoholism in the central nervous system.