宫颈癌中EZH2的表达与细胞增殖和血管生成的关系

Expression of EZH2 in cervical cancer and its relationship with cell proliferation and angiogenesis

目的检测果蝇zeste基因增强子2(EZH2)、增殖细胞核抗原(Ki67)和微血管密度在正常宫颈、宫颈上皮内瘤样变(CIN)和宫颈癌组织中的表达,探讨EZH2在宫颈癌发生发展中的作用及其与临床特征的关系。方法采用免疫组织化学法检测EZH2、Ki67和CD34在20例正常宫颈、35例CIN和50例宫颈癌组织中的表达,并分析EZH2与宫颈癌患者临床特征、Ki67和CD34之间的关系。结果 EZH2染色主要位于细胞核,在正常宫颈、CINⅠ、CINⅡ、CINⅢ和宫颈癌中的阳性表达阳性率分别为15.0%、20.0%、40.0%、66.7%和70.0%,呈上升趋势,组间比较有统计学差异(P<0.01)。EZH2与细胞增殖和血管生成、淋巴结转移呈显著相关性(P<0.05)。EZH2表达阳性率随分化程度的增高而降低,组间比较有统计学差异(P<0.05),但与患者的年龄、临床分期均无关(P>0.05)。结论 EZH2与肿瘤细胞的增殖活性有关,可能在宫颈癌的发展、浸润、转移和血管生成中起重要作用。

Objective To explore the role of enhancer of zeste homolog 2 （EZH2） in the pathogenesis and development of cervical cancer, and its relationship with clinical characteristics by examining EZH2, Ki67 and CD34 expressions in normal cervix, cervical intraepithelial neoplasia （CIN） and cervical cancer tissues. Methods Immunohistochemical staining was used to examine the expressions of EZH2, Ki67 and CD34 in 50 cases of cervical cancer, 35 cases of CIN and 20 normal cervical specimens. The relationship of EZH2 with clinicopathological characteristics, Ki67 and CD34 was analyzed. Results EZH2 was located at the cell nucleus, its positive rate showed a pattern of gradual increase from normal cervix （15.0%） to CINⅠ （20.0%）, CINⅡ （40.0%）, CINⅢ （66.7%） to invasive cervical carcinomas （70.0%） （P〈0.01）. The expression of EZH2 was significantly correlated with cell proliferation, angiogenesis and lymphatic metastasis （P〈0.05）. The positive rate of EZH2 decreased with the increase of differentiation degree, and there were significant differences between these groups （P〈0.05）. However, the expression of EZH2 was not related to patients＇ age or clinical stage （P〉0.05）. Conclusion EZH2 expression is significantly related to the proliferation of tumor cells and may play an important role in the development, infiltration, metastasis and angiogenesis of cervical cancer.