摘要
目的探讨柴胡皂甙d(saikosaponins-d,SSd)对实验性大鼠肝癌血管形成的影响及其作用机制。方法清洁级雄性SD大鼠100只,随机分为3组:模型组、SSd干预组和DMSO对照组。模型组经口灌注二乙基亚硝胺(diethylinitrosamine,DEN)溶液造模,每周5次至14周;DMSO对照组和SSd干预组在造模的同时分别每日给予腹腔注射SSd 1mg/kg和等体积量的DMSO。至18周末处死所有大鼠,记录大鼠的一般情况,取肝脏标本,HE染色观察组织病理学改变,免疫组织化学法和酶联免疫吸附法(ELISA)分别检测肝癌组织及血清中血管生成素2(Ang-2)和血管内皮细胞生长因子(VEGF)的表达。结果 SSd可抑制肿瘤形成,减轻肝脏损害,降低死亡率。免疫组化和ELISA法检测结果显示,SSd下调大鼠肝癌组织及血清中Ang-2和VEGF表达,与模型组和对照组相比差异具有统计学意义(P<0.05)。结论 SSd可以抑制实验大鼠肝癌发生及血管形成,下调Ang-2和VEGF的表达,这可能是其抑制肝癌的作用机制之一。
Objective To investigate the effect of saikosaponins-d （SSd） on experimental hepatocarcinogenesis in rats and its potential mechanisms. Methods One hundred SD male rats were randomly divided into model group, DMSO control group and SSd intervention group. The model group was administered intragastrically with diethylnitrosamine （DEN） （5 times/week） for 14 weeks. DMSO control group was simultaneously administered with the same dosage of DMSO as SSd group. Saikosaponin-d intervention group was injected intraperitoneally with SSd （1.0mg/kg） once a day for 18 weeks. All rats were killed at the end of week 18. The expressions of Ang-2 and VEGF in liver tissue and serum were measured by immunocytochemistry and ELISA. Results Liver injury was alleviated obviously and the death rate was reduced in SSd group. Immunocytochemistry and ELISA indicated
that Ang-2 and VEGF expressions were decreased significantly in SSd group compared with model and control groups （P〈0.05）. Conclusion SSd can inhibit angiogenesis of DEN-induced hepatocarcinogenesis and the mechanism may be related to the down-regulation of Ang-2 and VEGF expressions.
出处
《西安交通大学学报（医学版）》
CAS
CSCD
北大核心
2013年第5期664-668,共5页
Journal of Xi’an Jiaotong University（Medical Sciences）
基金
国家自然科学基金资助项目(No.30771895)~~
关键词
柴胡皂甙D
肝癌
血管生成素2
血管内皮细胞生长因子
saikosaponin-d （SSd）
hepatoma
angiopoietin-2
vascular endothelial cell growth factor （VEGF）
