“后肢跳台”方法在检查脑卒中大鼠运动功能缺陷中的应用

Hind limb jump onto countertop for testing motor functional deficit in rats after stroke

目的设计一种专门用于检测大鼠脑卒中后肢运动功能缺陷的行为学检查方法。方法对大鼠脑出血后1、7、14、21、28d后肢跳台方法的敏感性和稳定性与同侧转圈和横梁行走进行比较。结果脑出血大鼠同侧转圈持续时间较短、不超过7d,而横梁行走和后肢跳台症状持续时间均超过28d。三种检查相应阳性症状于脑出血1d最严重,以中重度为主,以后逐渐减轻。脑出血28d时,横梁行走和后肢跳台症状仍未完全恢复,但以轻中度为主。相比之下,后肢跳台试验的敏感性与同侧转圈和横梁行走相同,但稳定性优于同侧转圈,并与横梁行走等同。结论 "后肢跳台"试验是一种敏感、稳定、操作简单的可专用于脑卒中大鼠后肢运动功能缺陷的行为学检查方法。

Objective To design a behavioral test for detecting hind limb motor functional deficit in rats after stroke. Methods Rats’ sensibility and stability of hind limb jump onto countertop were compared with those on ipsilateral cycle and the beam walking 1, 7, 14, 21 and 28d after intracerebral hemorrhage （ICH）. Results The duration of ipsilateral cycle was less than 7 days, while that of hind limb jump onto countertop and beam walking was both more than 28 days. The corresponding positive symptoms of these three behavioral assessments were most serious 1d after ICH, dominated by moderate to severe, and gradually decreased afterward. 28d after ICH, the positive symptoms of hind limb jump onto countertop and beam walking were still not fully recovered, and dominated by mild to moderate. By comparison, the sensibility of hind limb jump onto countertop was equal to that of ipsilateral cycle and beam walking, while its stability was superior to that of ipsilateral cycle and equal to that of beam walking. Conclusion The experiment of hind limb jump onto countertop is a sensitive, stable and simple behavioral test to detect rat’s hind limb motor functional deficit after stroke.