PKAⅠ、HSP27及PTEN在口腔鳞癌组织中的表达及相关性研究

Expression and Significance of PKAⅠ,HSP27 and PTEN Protein in Oral Squamous Cell Carcinoma Significance

目的:探讨口腔鳞状细胞癌(口腔鳞癌,OSCC)癌组织中PKAⅠ、HSP27及PTEN的表达情况及临床生物学上的相关意义。方法:应用免疫组化SP法检测50例口腔鳞癌组织及20例正常口腔组织中的PKAⅠ、HSP27及PTEN的表达情况。结果:PKAⅠ、HSP27在病例组中的阳性表达率(分别为72%、80%),明显高于对照组(正常组)(分别为25%、40%)(P<0.05)。PTEN的阳性表达率在病例组中24%,明显低于正常对照组60%(P<0.05)。PKAⅠ在口腔鳞癌中的表达与HSP27呈正向相关(r=0.4048,P<0.05),PKAⅠ与PTEN呈负向相关(r=-0.3796,P<0.05),HSP27与PTEN呈负向相关(r=-0.7727,P<0.05)。结论:PKAⅠ、HSP27在口腔鳞癌的发生、发展过程中可能起着协同促进的作用,而PTEN在口腔鳞癌的发生、发展过程中可能起着拮抗抑制PKAⅠ、HSP27的作用。三者均可作为诊断鳞癌的依据。

Objective： To study the expression of PKA I , HSP27 and PTEN in oral squamous cell carcinoma and their clinical significance. Methods： The method of detectiving 50 patients with oral squamous cell carcinoma of PKA I , HSP27 and PTEN and 20 cases of normal oral tissues expression was SP method. Results： PKA I , HSP27 in the positive rate of OSCC （72%, 80%） were significantly higher than the normal oral mucosa（25%, 40%）. PTEN expression in normal oral mucosa 60.00% and in the positive rate of oral squamous cell carcinoma was 24% which had a significant difference between groups. There was positive correlation between the PKA Ⅰ and HSP27 expression. However, There were inverse correlation between PKA Ⅰ and PTEN expression and inverse correlation between HSP27αand PTEN expression. Conclusion： PKA I , HSP27 in oral squamous cell carcinoma may play an collaborative role in the event of the development process. PTEN may antagonize PKA I , and HSP27 expression. They all cart be used as the basis for diagnosis of oral squamous cell carcinoma.