DNA修复基因XRCC1 Arg399Gln多态性与新疆不同民族喉癌发生风险的相关性研究

Study on laryngeal cancer related on polymorphism of the Arg399Gln of XRCC1 DNA repair gene in different nationalities in Xinjiang

目的:探讨X射线交错互补修复基因1(XRCC1)Arg399Gln多态性与新疆不同民族喉癌发生风险的相关性。方法:采用病例组和对照组设计,选择新疆维、汉、哈民族60例喉鳞状细胞癌患者和120例体检正常的健康人,2组间年龄和性别进行匹配,应用Multiplex SNaPshot技术(多重单碱基延伸反应技术)方法检测DNA碱基切除修复基因XRCC1的Arg399Gln位点单核苷酸多态在病例组和对照组中的分布情况。结果:病例组中携带XRCC1c.399位点Arg/Gln(杂合型)及Gln/Gln(突变型)基因型的比例明显高于对照组(P<0.01)。其中汉、维、哈3个民族病例组XRCC 1c.399G1n/Gln基因型比例显著高于对照组(P<0.05),携带XRCC1c.399G1n/Gln基因型个体较携带XRCC1c.399Arg/Arg基因型的个体患喉鳞状细胞癌的风险分别升高了1.47倍(OR=1.47,95%CI为0.46～4.69),1.32倍(OR=1.32,95%CI为0.23～2.63),0.77倍(OR=0.77,95%CI为0.47～3.70)。结论:3个民族的XRCC1c.399多态性可能与喉癌遗传性有关联且有差异,XRCC1c.399位点Arg→Gln的突变将导致喉癌的发病风险升高。

Objective： To study genetic polymorphism of XRCCI Arg399Gln and lhe laryngeal cancer risk. Method： A case-control study was performed on 60 patients with laryngeal squamous cell carcinoma and 120 ran dom healthy control group. The two groups were matched by sex and age. Multiplex SNaPshol lechnic was used to explore polymorphism of DNA repair gene XRCC1 Arg＇~99Gln in distribution of patient with laryngeal squamous cell carcinoma and normal control. Result：The frequency of XRCClc. 399Arg/Gln--Gln/Gln genotypes in the case group was higher than that in the eontrol group（P --C. 0.05 ）. The expression of the three nations（chinese, uyhur, ka zak） was Respectively a 1.47,1.32,0.77 fold increased risk of laryngeal cancer for individuals arrying XRCClc. 399Arg/Glnq-Gln/Gln genotypes（OR= 1.47,95GCI=0. 46-- 4. 69）, compared with subjecls carryin Arg/Arg genolype. Conclusion： The polymorphism of XRCC1 Arg399Gln might be associated with the susceptibility of laryn- geal cancer. The mutation of XRCClc. 399 ArgGln might lead to a increased risk of laryngeal cancer.